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Study of the Effects of Lactoferrin in Reduction of Cyclophosphamide-induced Embryotoxicity: In Vitro Fertilization Study in Mouse
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| Parisa Saghaie1, Shapoor Hasan zadeh *2, Hasan Malekinejad1, Ali Shalizar jalali1 |
1- Dept of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2- Dept of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran , s.hasanzadeh@urmia.ac.ir |
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Abstract: (4113 Views) |
Introduction: Cyclophosphamide (CP) as a widely used chemotherapeutic agent causes several side effects including reproductive toxicities. The Objective of this study was to evaluate the possible protective effects of lactoferrin (LFN) with anti-inflammatory and antioxidant properties against CP-evoked reproductive toxicity in mouse.
Materials & methods: In this experimental study, 32 adult female NMRI mice were divided into 4 groups of 8. Cyclophosphamide was administered to two groups of mice at a dose of 200 mg/kg intraperitoneally on days 1 and 14. One of these groups received LFN at a dose of 0.2 mg/kg orally four hours after CP treatment. Vehicle-treated control group and LFN-only treated group were also included. In vitro fertilization was evaluated in all animals after 28 days.
Findings: Cyclophosphamide administration resulted in significant reduction in fertilization and blastulation rates. Co-administration of LFN with CP restored above-mentioned parameters toward normal values.
Discussion & conclusions: These findings indicate that LFN can have potential protective effects in CP-induced reprotoxicities.
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| Keywords: : Cyclophosphamide, Lactoferrin, Fertility, Mouse |
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Full-Text [PDF 690 kb]
(963 Downloads)
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Type of Study: Research |
Received: 2017/07/5 | Accepted: 2017/10/16 | Published: 2018/01/9
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