[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Contact us::
Site Facilities::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019

Search in website

Advanced Search
Receive site information
Enter your Email in the following box to receive the site news and information.
Registered in



:: Volume 26, Issue 4 (11-2018) ::
Journal of Ilam University of Medical Sciences 2018, 26(4): 1-12 Back to browse issues page
Antioxidant Activity of Glycyrrhiza glabra L. Extract and Protective Effect of its Leaf Extract on Ethanol-Induced Nephrotoxicity in Male Rats
Roya Karamian * 1, Mostafa Asadbeigy2 , Siamak Yari2
1- Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran , R_karamian@basu.ac.ir
2- Dept of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran
Abstract:   (5465 Views)

Introduction: Acute alcohol consumption leads to induction of lipid peroxidation in renal tissues, but its chronic consumption has moderate effects on biochemical and histological characteristics of this organ. Antioxidants have protective effects against ethanol-induced oxidative stress and tissue injury. The aim of this study was to assess antioxidant activity of Glycyrrhiza glabra leaf and stem extracts and the protective effect of its leaf extract on ethanol-induced nephrotoxicity.
Materials & methods: Total phenol and flavonoid contents of leaf and stem extracts of G. glabra were measured by Folin Ciocalteu and AlCl3 assays, respectively. Antioxidant activity of both extracts was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. In addition, protective effect of the leaf extract was assessed using biochemical and histological analyses of renal tissues of male Wistar rats, which were divided into four groups including group 1 or control (received 1 ml distilled water daily), group 2 or ethanol group (received 1 ml of 50% ethanol daily), group 3 or ethanol + leaf extract group (received 1 ml of 50% ethanol + 500 mg/kg leaf extract daily), and group 4 (received 500 mg/kg of leaf extract daily). All treatments are performed through intragastric administration. Biochemical and histological analyses were used for the evaluation of nephrotoxicity. For histological study, the samples were stained with Hematoxylin-Eosin and examined by light microscopy. Finally, all the data were analyzed by SPSS (Ver. 20) and grouped by Duncan's Multiple Range Test at P <0.05 level.
Findings: There was no significant difference between total phenol contents of the stem and leaf extracts. However, the stem extract showed a higher total flavonoid content than the leaf extract. Also, both the extracts showed higher antioxidant activities (86-93%) than that of ascorbic acid (71%). Results from biochemical analysis indicated a significant increase in superoxide dismutase (SOD) activity and H2O2 content in the renal tissues of ethanol-treated rats in comparison with other groups; however, there were no significant changes in total protein and malondialdehyde (MDA) contents. Results from histological examination showed that alcohol consumption intensity injured kidney tissues, which was effectively moderated by the studied extract.
Discussion & Conclusions: Results from the present study showed that G. glabra extract has biological activity and can be used in future as a new natural antioxidant in food and drug industries.

Keywords: Glycyrrhiza glabra L., Antioxidant activity, Ethanol, Nephrotoxicity, Phenol, Rats
Full-Text [PDF 826 kb]   (4099 Downloads)    
Type of Study: Research | Subject: biology
Received: 2017/04/28 | Accepted: 2017/07/8 | Published: 2018/11/15
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Karamian R, Asadbeigy M, Yari S. Antioxidant Activity of Glycyrrhiza glabra L. Extract and Protective Effect of its Leaf Extract on Ethanol-Induced Nephrotoxicity in Male Rats . J. Ilam Uni. Med. Sci. 2018; 26 (4) :1-12
URL: http://sjimu.medilam.ac.ir/article-1-4282-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 4 (11-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.16 seconds with 41 queries by YEKTAWEB 4654