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:: Volume 25, Issue 6 (3-2018) ::
Journal of Ilam University of Medical Sciences 2018, 25(6): 130-140 Back to browse issues page
Evatuation of Drug susceptibility of Aspergillus species Isolated from ICU of Hospitals in Invitro
Ayatollah Nasrolahiomran *
Dept of Mycology, Faculty of Medicine, Islamic Azad University, Tonkabon Branch,Tonkabon, Iran , ayqt51@yahoo.co.in
Abstract:   (4743 Views)

Introduction: Invasive aspergillosis is the most threatening disease inimmunoco-mpromised patients which has the highest morbidity and mortality rate among invasive fungal infections in the hospitals. The aim of present study was to assess antifungal susceptibilitytesting versus Aspergillus spp isolated from the hospitals environment.
Materials & Methods: After collecting 160 plates containing Sabouraud dextrose agar from the air and the environment of hospital's ICUs (intensive care units), the phenotypic and molecular identification of the colonies was performed for the identification of Aspergillusspp. After DNA extraction, the molecular identification was carried out using universal fungal primers (ITS gene) and DNA sequencing. Antifungal susceptibility testing was performed using the CLSI broth microdilution (M38-A2) method for Aspergillus isolates.
Findings: Out of 160 hospital environmental samples, 11Aspergillus species were obtained. The eleven Aspergillus spp. were identified by sequencing as: 5 A. flavus, 3 A. sydowii, 1 A. fumigates and 2A. Oryzae. Our antifungal susceptibility testing results indicated that A. sydowii and A. fumigatus were sensitive to Amphotericin and Voriconazole and also were resistant to Itraconazole. A. sydowii was resistant to Caspofungin while A.fumigatus was sensitive to this drug. A. flavus was susceptible to all the drugs.
Discussion & Conclusions: There were a number of reasons including delayed diagnosis, lack of appropriate curing, and the existence of various diseases and neutropenia which could lead to the high mortality rate of patients with Aspergillosis, especially in patients of hospital's ICUs.

Keywords: Drug susceptibility, Aspergillus SP. ICU
Full-Text [PDF 661 kb]   (2355 Downloads)    
Type of Study: Research |
Received: 2017/03/15 | Accepted: 2017/11/15 | Published: 2018/03/13
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Nasrolahiomran A. Evatuation of Drug susceptibility of Aspergillus species Isolated from ICU of Hospitals in Invitro . J. Ilam Uni. Med. Sci. 2018; 25 (6) :130-140
URL: http://sjimu.medilam.ac.ir/article-1-4199-en.html

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Volume 25, Issue 6 (3-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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