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:: Volume 24, Issue 5 (12-2016) ::
Journal of Ilam University of Medical Sciences 2016, 24(5): 175-184 Back to browse issues page
Rapid Detection and Prevalence of Cytomegalovirus in Infants under Three Weeks by PCR and Real Time PCR
Reza Yari * 1, Ali Javadi2 , Abbas Morovvati3 , Talayeh Shakeri3
1- Dept of Biology, Faculty of Sciences, Islamic Azad University, Borujerd Branch, Borujerd, Iran , Abbasmorovvati@gmail.com
2- Dept of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3- Dept of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran
Abstract:   (6944 Views)

Introduction: Most of the newborns’ diseases transfer to them through their mothers. Cytomegalovirus (CMV) is the most prevalence infections which transfer from mother to embryo. CMV is the cause of congenital infection and is known as spontaneous abortion in mothers. This virus can cross the placenta and cause clinical signs in embryo and infant. The aim of this study was to develop a PCR and Real Time PCR for diagnosis of Cytomegalovirus using molecular tests.

Materials & methods: In this study, out of 100 infants with clinical signs (under 3 weeks) who referred to Qom and were suspected to infection with CMV. The urine samples were studied for existence of DNA for using PCR and Real Time PCR methods and using specialized primers and probe.

Findings: Out of 100 newborn, DNA of CMV was excreted through urine. 58 newborns have CMV DNA in their urines. The results with 2 methods have similar PCR and Real Time PCR. The CMV DNA was not found in 19 newborns.

Discussion & conclusions: The results show that the molecular tests are influential for fast diagnosis especially tracking viral DNA using PCR method. Also on the strength of this research, the importance of this study and submitting solutions for fast and accurate diagnosis of this Virus in newborns immediately after delivery is necessary for treatment and preventing this Virus.

Keywords: Cytomegalovirus, PCR, Real Time PCR, Urine
Full-Text [PDF 802 kb]   (5605 Downloads)    
Type of Study: Research | Subject: biology
Received: 2016/09/10 | Accepted: 2016/11/9 | Published: 2017/01/18
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yari R, Javadi A, Morovvati A, shakeri T. Rapid Detection and Prevalence of Cytomegalovirus in Infants under Three Weeks by PCR and Real Time PCR. J. Ilam Uni. Med. Sci. 2016; 24 (5) :175-184
URL: http://sjimu.medilam.ac.ir/article-1-3801-en.html


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Volume 24, Issue 5 (12-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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