Introduction: Candidal vulvovaginitis occurs in female genital by the over growth of candida especially candida albicans. This infection may be resistant to therapy and occasionally becomes chronic. In some patients this form of infection is recurrent. Candida species are the most important factors in development of fungal infection in human beings and animals. According to the daily increase of drug resistance, attempts are important for providing anti-fungal and anti-microbial drugs. With the increasing consumption of medical plants for medical treatment, this topic of complementary medicine found a unique place. This study compares the concentration effects of Cichorium intybusL. and Matricaria chamomilla L.  hydroalcoholic extract and the mixed with Nystatin on the isolated Candida albicans from Vaginal Candidiasis and standard under in vitro conditions. 
 
Materials & Methods: This experimental study assessed the antifungal effects of two plant extracts on candida albicans. Cichorium intybus L and Matricaria chamomilla L. were selected based on the previous researches and traditional usages.   C.Albicans was cultured on sabouraud dexterose agar containing chloramphenicol. On each plate, one plant extract disc, one nystatin disc (as positive control), and one blank disc (as negative controls) were placed. The mean diameter of non-growth halo around each plant extract was compared with the mean diameter of non-growth halo of positive control discs after 24 hours. Data was analysed by ANOVA statistical test (P<0.05).
 
Findings: There was a significant difference between the diameter of non-growth halo around the plant extracts and Nystatin discs. The diameter of non-growth halo in nystation discs (30 mm) was more than that for Matricaria chamomilla L. discs (21 mm). Non-growth halo in discs (28 mm) nystation were also more than Cichorium intybus L. discs (17 mm) (p<0.05). As well as the diameter of non-growth halo in Combination Matricaria chamomilla L. and Nystatin discs(47 mm) was more than that for nystatin Matricaria chamomilla L. discs(21 mm) and the diameter of non-growth halo in Combination Cichorium intybusL, and Nystatin discs(45 mm) was more than that for nystatin Cichorium intybusL discs(17 mm).
 
Discussion & Conclusions: Cichorium intybusL and Matricaria chamomilla L.  exhibited antifungal effects on C. Albincans But the effect of Matricaria chamomilla L.  on the lack of growth of Candida Albicans strains was greater than that in Cichorium intybusL.