Introduction: Polycystic ovary syndrome (PCOS) as an endocrine disorder, the most common cause of infertility in couple is considered, for be a better understanding of the pathogenesis of this syndrome, the animal models is needed. This study was conducted with the aim of polycystic ovary induction by dehydroepiandrostenedione in mice.
 
Materials & methods: To induce PCOS phenotype of immature mice (approximately 21-22 days) in both control and experimental groups of 12 mice were studied. in the experimental group for 21 days with a daily injection of dehydroepiandrostenedione were treated intraperitoneally. The solvent of dehydroepiandrostenedione was injected to the control group. For evaluate changes in ovarian tissue, histomorphometry methods were used.
 
Findings: The use of dehydroepiandrostenedione in order to create a model of polycystic ovary syndrome causes a significant reduction in the number of antral follicles and the corpus luteum and increase the number of follicles primordial, primary and preantral and cysts in comparison to the control group (P<0.05). Mice treated with dehydroepiandrostenedione in compared with the control group respectively showed a significant increase in the size of the follicles (P<0.001), a significant increase in the thickness of the theca (P<0.001) and a significant decrease in granulosa layer thickness (P<0.001).
 
Discussion & Conclusions: Based on the results of this study, we can conclude that dehydroepiandrostenedione can induce polycystic ovary in mice.