Introduction: Using nutritional supplements is proposed to avoid the stresses-induced by physical activity, delayed fatigue and maintenance of performance during competition. The aim of this study was to explore the effect of combined supplement Neurobion on Creatine Kinase (CK) and Lactate Dehydrogenase (LDH) enzymes levels following a session of exhaustive exercise in amateur men boxers.

Materials & methods: Twelve men boxer with an average age of19.1±2 years, weight 71.3±6.2 kg and percentage of body fat 8.9±0.7 participated in this study. In two consecutive weeks, they consumed Neurobion supplement and placebo (distilled water) into5 ml ampoule and then carried out exhaustive exercise boxing. Exercise implemented with fists swiping to punch bag in 3 rounds of 3 minute with 1 minute rest intervals between rounds with maximum intensity and competitive. To measure the levels of LDH and CK induced by muscle cell damage, blood samples were taken in four stages before, immediately, and 2and6hours after exercise.

Findings: The results showed that consuming combination of vitamin Neurobion had a significant effect on serum levels of LDH and CK in different stages of exercise (p=0.001).With intake supplement, the amounts of CK in2and6hours, and LDH in the immediately after the exercise, compared with placebo group had a significant reduction (p<0.05).

Discussion & conclusions: It is concluded that, using combination of vitamin supplement Neurobion by maintaining molecular structure of cells and sarcomere, as well as muscle cell membrane integrity, cause reduced amounts of LDH and CK enzymes in the amateur boxers after exhaustive exercise.