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Journal of Ilam University of Medical Sciences 2016, 24(1): 154-161 Back to browse issues page
Molecular Identification of Escherichia coli EPEC Isolated from Children Under the Age of 5 Years by Multiplex PCR in Kermanshah
Zahra Nazari1, Gholamaliye Moradli *2, Bita Bakhshi3
1- Saveh Branch, Islamic Azad University
2- Saveh Branch, Islamic Azad University , Moradli.mic@gmail.com
3- Tarbiat Modares University
Abstract:   (6688 Views)

Introduction: Infection Diarrhea is one of the most common causes of death in children. Each year, approximately two million people die worldwide of diarrhea. Entropathogenic E. coli is the most common cause of the acute and chronic diarrhea in children in developing countries. The aim of this study was to evaluate the prevalence of the  EPEC pathotype  genes and their antibiotic resistance among isolated bacteria from suspected cases of diarrhea children under age of 5 years.

Materials & methods: A total of 150 fecal samples of diarrheic children referred to Kermanshah hospital were collected during 5 months. 55 isolates were confirmed as E.coli by biochemical and microbiological tests. Antimicrobial susceptibility were tested by disk diffusion method according to CLSI guidelines. Multiple PCR assay was used to identify pathotype geneseae  and BFP.

Findings: E.coli isolates were reported resistant to imipenem (100%) and tetracycline (73.34%) and were sensitive to ciprofloxacin (100%) and gentamicin (96.66 %). Results of M-PCR showed that three isolated (5.4%) have eae gene.

Discussion & Conclusions: Because of the importance of E.Coli as the main cause of diarrhea in children in developing countries, and due to increasing consumption and resistance to antibacterial agents, it is a threat for the health of patients.  This  study shows that the incidence of Atypical E.coli is more than typical prevalence.

Keywords: Escherichia coli, Disk diffusion, EPEC, Multiplex PCR
Full-Text [PDF 739 kb]   (3382 Downloads)    
Type of Study: Research | Subject: Medical microbiology
Received: 2015/06/19 | Accepted: 2015/08/18 | Published: 2016/05/14
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nazari Z, Moradli G, Bakhshi B. Molecular Identification of Escherichia coli EPEC Isolated from Children Under the Age of 5 Years by Multiplex PCR in Kermanshah. Journal of Ilam University of Medical Sciences 2016; 24 (1) :154-161
URL: http://sjimu.medilam.ac.ir/article-1-2805-en.html


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Volume 24, Issue 1 (4-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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