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Assessment of Brucella Abortus Lipopolysaccharide
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Abstract: (15817 Views) |
| Abstract
Introduction: Lipopolysaccharide (LPS) of Brucella abortus is important in diagnosing brucellosis as well as in developing a subunit vaccine against this disease. Biological evaluation of B. abortus LPS and it's protective potentials have been the goal of this research.
Meterials & Methods: Brucella abortus LPS was extracted by n-butanol ,then purified by ultracentrifugation and detoxified by alkaline treatment. Toxicity rate of LPS and detoxified form of –LPS (D-LPS) using LAL( Limulus Amoebocyte Lysate) method, and pyrogenicity in rabbit were investigated . The immunological evaluation in animal model was also performed.
Findings: Purified LPS from B.abortus by butanol extraction was proven to have a <2 %(wt/wt) contamination with protein and a <1%(wt/wt) contamination with nucleic acids. Pyrogenicity test of B.abortus LPS (10 μg/ml) and E.coli LPS (0.5 μg/ml) was positive ,but negative for D-LPS (10, 50μg/ml). In LAL test , 10 ng/ml of D-LPS was negative , but 0.04ng/ml of B.abortus LPS was positive and endotoxin unit of B.abortus LPS was less than E.coli LPS. Antibody titer of LPS group was higher than that of D-LPS group. The difference of protection power among LPS group, D-LPS group compared with negative control was significant (p≤0.05), In addition, the difference of protection ratio between LPS and D-LPS groups was not significant.
Conclusion: As shown by the results that D-LPS toxicity is severely decreased, we can use as many more as B.abortus LPS for stimulating immunity system. Besides, ability of B.abortus LPS is likely much less than the LPS from E.coli to evoke endotoxic shock, and can be used directly as an immunogen. In addition, protection of LPS and D-LPS probably is due to humoral important role in secondary infection of brucellosis. |
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| Keywords: Lip polysaccharide (LPS), Brucella abortus, Detoxification, LAL, Rabbit Pyrogenic Test. |
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Full-Text [PDF 457 kb]
(3837 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2009/01/27 | Accepted: 2015/04/20 | Published: 2015/04/20
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