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:: Volume 23, Issue 1 (4-2015) ::
Journal of Ilam University of Medical Sciences 2015, 23(1): 143-150 Back to browse issues page
The survey of Microbial Contamination Prevalance and the Effective Factors in ILam University of Medical Sciences Student’s Toothbrushes
Kobra Heidarzadi1 , Farid Azizi Jalilian * 2, Ali Reza Rekabi1 , Razieh Amini3 , Iraj Pakzad1 , Morovat Taherikalani1 , Ali Hematian1 , Zahra Ghobadian1 , Maryam Bouchani1
1- Ilam University of Medical Science
2- Hamedan University of Medical Sciences , azizijalilian@yahoo.com
3- Hamedan University of Medical Sciences
Abstract:   (8569 Views)

The most common method of oral hygiene is tooth brushing. However, occasionally brushing becomes a risk factor for health. The main reason is getting infected with various microorganisms. The purpose of this study was to investigate bacterial and fungal contamination of toothbrushes students, and factors affecting the contamination.
 Materials & Methods: Ninety five brushes were assessed in this study. Samples were incubated in test tubes containing nutrient broth for twenty-four hours. Then the microorganisms were isolated using specific nutrient medium containing Blood agar, Chocolate agar MacConkey and sabouraud dextrose agar. The inoculated plates were incubated for 24 to 48 hours at 37 C. The identification of micro-organisms was performed based on biochemical tests.
 Findings: The infection rate was more than 40% of the subject staphylococcus, Niesseria, Diphteroids,Actinomycetes and Enterobacteriacae family members were isolated from samples. Microbial contamination was higher in women than men (P<0.05).There was found correlation between the number of microorganism on brush with time of use, using mouthwash and gender (P<0.05). There was statistically significant relation between cocci microorganisms’ infections with sexes as well as between bacillus infections with capped brush.
Discussion & Conclusion: Based on results, high incidence of bacterial contamination observed in brushes, therefore the proper use, maintenance and timely replacement of toothbrush play an important role in reducing pollution and consequently decreasing oral disease.

Keywords: Oral hygiene, Microbial contamination, Toothbrush
Full-Text [PDF 571 kb]   (3887 Downloads)    
Type of Study: Research | Subject: Bacteriology
Received: 2015/03/9 | Accepted: 2015/04/15 | Published: 2015/05/18
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Heidarzadi K, Azizi Jalilian F, Rekabi A R, Amini R, Pakzad I, Taherikalani M, et al . The survey of Microbial Contamination Prevalance and the Effective Factors in ILam University of Medical Sciences Student’s Toothbrushes. J. Ilam Uni. Med. Sci. 2015; 23 (1) :143-150
URL: http://sjimu.medilam.ac.ir/article-1-2582-en.html

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Volume 23, Issue 1 (4-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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