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:: Volume 23, Issue 6 (1-2016) ::
Journal of Ilam University of Medical Sciences 2016, 23(6): 142-151 Back to browse issues page
Antioxidant Properties of Brevinin-2R Peptide Conjugated with Cerium Oxide Nanoparticle
Masoud Homayouni1 , Ahmad Asoodeh * 2, Mohammad Mashreghi3 , Mahere Rezazade Bazaz4 , Majid Darroudi4 , Reza Kazemi Oskuee4
1- Islamic Azad University, Mashhad Branch
2- Ferdowsi University of Mashhad , Asoodeh@um.ac.ir
3- Ferdowsi University of Mashhad
4- Mashhad University of Medical Sciences
Abstract:   (7505 Views)

Introduction: Peptide Brevinin-2R has been isolated from frog Ranaridibunda skin secretions. This peptide has anti-bacterial and anti-cancer properties. Cerium oxide (CeO2, ceria) is a lanthanide metal oxide which has the ability to participate in the reduction and oxidation reactions. In this study, antioxidant properties of cerium oxide nanoparticles conjugated with the peptide Brevinin 2R were measured.

Materials & methods: Cerium oxide nanoparticles were synthesized in starch solution. Then, the surface of cerium oxide nanoparticles was functionalized with amine and conjugated with peptide Brevinin-2R. Furthermore, antioxidant activity of cerium oxide nanoparticles conjugated with peptide was evaluated by scavenging of DPPH, ABTS, and hydroxyl and superoxide radicals. Also, inhibition of linoleic acid oxidation was investigated.

Findings: The results showed that cerium oxide nanoparticles conjugated with peptide inhibits oxidation of linoleic acid and also served as radical scavenging of DPPH (IC50=0.2 mg/ml), ABTS (IC50=0.54 mg/ml), superoxide (IC50=0.078 mg/ml) and hydroxyl (IC50=0.1 mg/ml).

Discussion & Conclusions: The results showed that cerium oxide nanoparticles conjugated with Brevinin-2R peptide isolated from frog skin had a strong antioxidant activity. Its antioxidant activity is probably because of presence of amino acids such as cysteine, phenylalanine, and hydrophobic amino acids including leucine and alanine. The antioxidant properties of peptide conjugated nanoparticle could be due to the presence of cerium oxide.

Keywords: Peptide, Antioxidant activity, Cerium oxide nanoparticles
Full-Text [PDF 605 kb]   (3774 Downloads)    
Type of Study: Research | Subject: biochemistry
Received: 2014/12/21 | Accepted: 2015/06/6 | Published: 2016/01/17
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homayouni M, Asoodeh A, Mashreghi M, Rezazade Bazaz M, Darroudi M, Kazemi Oskuee R. Antioxidant Properties of Brevinin-2R Peptide Conjugated with Cerium Oxide Nanoparticle. J. Ilam Uni. Med. Sci. 2016; 23 (6) :142-151
URL: http://sjimu.medilam.ac.ir/article-1-2389-en.html

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Volume 23, Issue 6 (1-2016) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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