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Comparison of in vitro inhibitory effects of different extracts of Scrophularia striata plant on Staphylococcus aureus, Pseudomonas aeruginosa and Helicobacter pylori
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M Nazari   , I Pakzad * 1, A Maleki , A Hematian |
| 1- , pakzad-i2006@yahoo.com |
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Abstract: (11771 Views) |
| Introduction: The most important nosocomial infectious agents are Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aerug-inosa), and Helicobacter pylori (H. pylori). An alternative way for treatment of bacterial infection is herbal therapy. The aim of this study was to evaluate the inhibitory effects of alcoholic and water extracts of Scrophularia striata (S. striata) plant on S. aureus, P.aer-uginosa and H. Pylori at in vitro condition.
Materials & Methods: Water and alcoholic extracts of S. striata plant were prepared. Inhi-bitiory effect of these extracts was examined on S. aureus and P.aeruginosa in Mueller-Hi-nton agar medium. MIC and MBC of the ext-racts was determined in TSB medium by ma-cro dilution method. MIC and MBC of H. Pylori was determined by agar diffusion me-hod.
Findings: Inhibitory zone of water extraction for S. aureus and P. aeruginosa was 16 mm and 24 mm, respectively. Inhibitory zone of etha-nol extract for S. aureus and P. aeruginosa w-as 11 mm and 12 mm and that of methanol ext-ract was 12 mm and 16 mm, respectively.
The chloroform extract had not any inhibitory effect. The MIC of water extract of S. striata for S. aureus, P. aeruginosa and H. Pylori were 15µg/ml, 20µg/ml, and 5µg/ml, respectively. Also, the figures of MBC were 10µg/ml, 40µ-g/ml and 50µg/ml, respectively.
Discussion & Conclusion: Water and methanol extracts of S. striata had better inhibitory effect then other extracts. The best effect was belo-nged to the 5-hour boiled extract of the plant. Given to the MIC and MBC values, water extr-act of S. striata may be a candidate for herbal therapy in bacterial infections |
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| Keywords: Herbal, Scrophularia striata, extra-ction, MIC |
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Full-Text [PDF 340 kb]
(2721 Downloads)
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Type of Study: Research |
Subject:
physiology
Received: 2014/07/21 | Accepted: 2014/07/21 | Published: 2014/07/21
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