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Optimization of Effective Parameters on Cut-off of Rapid Detection System of Morphine Based on Lateral Flow Assay (LFA) Method
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H Shamlo ahmad   , M Heat , H Rashedi , Gh r Ovlad , A m Latifi * 1, R Haidari moghadam |
| 1- , amlatify@yahoo.com |
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Abstract: (10497 Views) |
| Introduction: Lateral flow assay method is a test for rapid detection of morphine that h-as many applications in identification of ad-dicts. However, detection level of the sys-tem has limited its application. This rese-arch examined how to improve the detec-tion level and sensitivity of this system.
Materials & Method: pH of conjugation, OD of conjugated material and concentr-ation of morphine-BSA in the test band w-ere effective on the detection level of this system. To determine optimum pH, a com-mercial kit (Gold in a box) was used. By applying various concentrations of morp-hine solution, and considering a constant concentration of morphine-BSA, the opt-imal OD was set. Then, the optimal conc-entration of morphine-BSA was obtained for a fixed amount of OD. Effect of block-ing the conjugated pad and addition of su-gar to the conjugated substance were also studied.
Findings: Optimum values of pH and OD was pH=8.4 and OD=1. Optimum conce-ntration of morphine-BSA in the test band was obtained to be 0.5 mg/ml. Cut-off value of optimized kit attained to 25 ng/ml. After comparing the results with a standard kit, sensitivity and specificity of the optimized kits were 100 % and 99% , respectively.
Discussion & Conclusion: Finding and opt-imizing the effective factors on the perf-ormance of diagnostic lateral flow method can be an effective strategy to increase the sensitivity and reduce the detection level of this system. |
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| Keywords: Morphine detection, OD Of co-njugated material, pH of conjugation, LFA system, optimization |
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Full-Text [PDF 425 kb]
(4787 Downloads)
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Type of Study: Research |
Subject:
Environmental health
Received: 2014/07/6 | Accepted: 2014/07/6 | Published: 2014/07/6
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