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:: Volume 22, Issue 4 (9-2014) ::
Journal of Ilam University of Medical Sciences 2014, 22(4): 109-122 Back to browse issues page
Comparison phenotypic methods with mecA gene analysis for the detection of methicillin/oxacillin resistance in clinical isolates of staphylococcus aurous in sanandaj city
D Amini1 , F Keshavarzi * 2, A Fatahi rad
1- Department of biology, Kurdistan Science and Research branch, Islamic Azad university, sanandaj, Iran
2- Department of biology, Sanandaj Branch, Islamic Azad university, sanandaj, Kurdistan, Iran , gol.keshavarzi@gmail.com
Abstract:   (8422 Views)
Abstract Introduction: Staphylococcus aureus is one of the important agents of many infections in hospitals and society. Increasing Staphylococcus aureus resistance to antibacterial drugs is one of the major health concerns, therefore studying antibiotic resistance of Staphylococcus aureus is very important and it has a main role in preventing creation of resistant strains. Materials and Methods: The antibiotic resistance of 150 Staphylococcus aureus isolates from various clinical specimens was determined by disk agar diffusion (DAD) and polymerase chain reaction (PCR) methods. Statistical analysis of data has performed by SPSS and Microsoft Office Excel software. Findings: Using the DAD method, 77/33% (116/150) of the Staphylococcus aureus isolates were resistant to Methicillin and 47/33% (71/150) were resistant to oxacillin. The results indicated that primer MR3,4 was more appropriate than primer MR1,2 for the detection of mecA gene in MRSA. Discussion and Conclusion: The results of this study can provide guidance for physicians toward a more appropriate treatment of Staphylococcus aureus infections in Iran, thereby preventing the emergence of further antibiotic resistance among Staphylococcus aureus. Our results also revealed the need for further investigations using a higher number of specimens representing a wider variety of locations to determine the antibiotic resistance patterns in our state more precisely.
Keywords: Staphylococcus aureus, Antibiotic Resistance, PCR.
Full-Text [PDF 672 kb]   (2243 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2014/03/29 | Accepted: 2014/05/28 | Published: 2014/09/8
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Amini D, keshavarzi F, fatahi rad A. Comparison phenotypic methods with mecA gene analysis for the detection of methicillin/oxacillin resistance in clinical isolates of staphylococcus aurous in sanandaj city. J. Ilam Uni. Med. Sci. 2014; 22 (4) :109-122
URL: http://sjimu.medilam.ac.ir/article-1-1682-en.html

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Volume 22, Issue 4 (9-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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