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:: Volume 21, Issue 7 (2-2014) ::
Journal of Ilam University of Medical Sciences 2014, 21(7): 191-195 Back to browse issues page
Evaluating Human Serum Albumin Structure and Stability in the Presence of DTAB
P Vasoghi *1, N Ahmadi, M Shahani, E Bahadori monfared, M Azizi
1- , padinavm@gmail.com
Abstract:   (9605 Views)
Introduction: Human serum albumin with its singular structure acts as a carrier mo-lecule that can attach to a variety of sub-stances. Any structural or functional chan-ges in albumin are accompanied with unde-sirable outcomes. In the present research, attachment of some ligands such as det-ergents (DTAB) and sugars (glucose) to albumin were evaluated at temperatures 27°C and 42°C. Materials & Methods: Tris-buffer solusion for albumin with a pH close to the pH of body was prepared. To denature albumin, hydrochloric acid was used. UV spectero-photometric technique was applied to eval-uate denaturation process. Findings: With increment of DTAB conc-entration the maximum optical absorption of albumin decreased. Glucose concent-ration at 400mg/dl level caused a profound change in the optical absorption of albumin that might affect the structure of the mo-lecule. Discussion & Conclusion: Glucose elevate-on has deleterious effects and deteriorates the function of many molecules in blood. The effects may exert on hormones, drug transportation, necessary ions and elements
Keywords: Human serum albumin, DTAB cation, glucose, thermal denaturation, UV spectrophotometry
Full-Text [PDF 447 kb]   (3545 Downloads)    
Type of Study: Research | Subject: anatomy
Received: 2014/02/18 | Accepted: 2014/02/18 | Published: 2014/02/18
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vasoghi P, ahmadi N, shahani M, bahadori monfared E, azizi M. Evaluating Human Serum Albumin Structure and Stability in the Presence of DTAB. Journal of Ilam University of Medical Sciences 2014; 21 (7) :191-195
URL: http://sjimu.medilam.ac.ir/article-1-1596-en.html


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Volume 21, Issue 7 (2-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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