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:: Volume 21, Issue 7 (2-2014) ::
Journal of Ilam University of Medical Sciences 2014, 21(7): 191-195 Back to browse issues page
Evaluating Human Serum Albumin Structure and Stability in the Presence of DTAB
P Vasoghi * 1, N Ahmadi , M Shahani , E Bahadori monfared , M Azizi
1- , padinavm@gmail.com
Abstract:   (10009 Views)
Introduction: Human serum albumin with its singular structure acts as a carrier mo-lecule that can attach to a variety of sub-stances. Any structural or functional chan-ges in albumin are accompanied with unde-sirable outcomes. In the present research, attachment of some ligands such as det-ergents (DTAB) and sugars (glucose) to albumin were evaluated at temperatures 27°C and 42°C. Materials & Methods: Tris-buffer solusion for albumin with a pH close to the pH of body was prepared. To denature albumin, hydrochloric acid was used. UV spectero-photometric technique was applied to eval-uate denaturation process. Findings: With increment of DTAB conc-entration the maximum optical absorption of albumin decreased. Glucose concent-ration at 400mg/dl level caused a profound change in the optical absorption of albumin that might affect the structure of the mo-lecule. Discussion & Conclusion: Glucose elevate-on has deleterious effects and deteriorates the function of many molecules in blood. The effects may exert on hormones, drug transportation, necessary ions and elements
Keywords: Human serum albumin, DTAB cation, glucose, thermal denaturation, UV spectrophotometry
Full-Text [PDF 447 kb]   (3742 Downloads)    
Type of Study: Research | Subject: anatomy
Received: 2014/02/18 | Accepted: 2014/02/18 | Published: 2014/02/18
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vasoghi P, ahmadi N, shahani M, bahadori monfared E, azizi M. Evaluating Human Serum Albumin Structure and Stability in the Presence of DTAB. J. Ilam Uni. Med. Sci. 2014; 21 (7) :191-195
URL: http://sjimu.medilam.ac.ir/article-1-1596-en.html

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Volume 21, Issue 7 (2-2014) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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