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:: Volume 23, Issue 1 (4-2015) ::
Journal of Ilam University of Medical Sciences 2015, 23(1): 112-119 Back to browse issues page
The Effect of DMSO in Induction of Differentiation of Cardiomyocytes from Embryonic Carcinoma Cells P19 in Invitro
Majid Malekzadeh Shafaroudi * 1, Nourolah Rezaei2 , Saeid Abedian kenari2 , Raha Setorg2 , Mostafa Latifpour2 , Hatef Ghasemi Hamidabadi2
1- Mazandaran Medical Sciences University , malek1344@gmail.com
2- Mazandaran Medical Sciences University
Abstract:   (7761 Views)

Introduction: In the present study, the ability of P19 embryonic carcinoma cell differentiation to cardiomyocytes in different concentrations of DMSO was evaluated.
Materials & Methods: In order to providing differentiated P19 cells, embryonic-like objects (Embryoid Bodies, EBs) formed during 2 days through hanging drops method. Then EBs induced for 18 days under the different concentrations of DMSO (0.1%, 0.5%, 1%, 1.5% and 2%). The rate of cardiomyocytes beating was counted with three days interval in the various groups by inverse microscope. Immunocytochemistry was used to illustrate expression of F-actin protein.
 Findings: Many morphological changes occurred in cardiomyocytes derived from P19 especially in DMSO 0.5% group, so a remarkable increase in the size of the cytoplasm and cytoplasmic processes was observed. The results revealed that the maximum beating frequency per minute was observed in DMSO 0.5% group concentration in 2+12 days (2 days for making emberyonic bodies, and 12 days after differentiation induction). In addition, only cardiomyocytes induced differentiation by DMSO 0.5% concentration expressed F-actin which was not expressed in the other concentrations.
 Discussion & Conclusion: The findings of this study suggest that, firstly, the most effective concentration in the process of cardiogenesis induction is 0.5%. Secondly, P19 carcinoma cells are desirable and susceptible to differentiate cardiomyocytes.

Keywords: P19 Cells, Cardiomyocytes, F-actin protein, Embryoid bodies, DMSO
Full-Text [PDF 705 kb]   (1958 Downloads)    
Type of Study: Research | Subject: gardiologist
Received: 2014/02/10 | Accepted: 2014/06/9 | Published: 2015/05/17
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Malekzadeh Shafaroudi M, Rezaei N, Abedian kenari S, Setorg R, Latifpour M, Ghasemi Hamidabadi H. The Effect of DMSO in Induction of Differentiation of Cardiomyocytes from Embryonic Carcinoma Cells P19 in Invitro. J. Ilam Uni. Med. Sci. 2015; 23 (1) :112-119
URL: http://sjimu.medilam.ac.ir/article-1-1580-en.html

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Volume 23, Issue 1 (4-2015) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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