Introduction: Fatigue is an unpleasant symptom including a spectrum from feeling weak to becoming fatigue which interferes in individuals’ abilities in doing perfo-rmance. There is no valid and reliable instrument to measure instructors’ fatigue in Iran. Therefore, this study aimed to tr-anslate and psychometricallyevaluate “Mu-ltidimensional Symptoms Fatigue Inven-tory-Short Form”. Materials & Methods: This descriptive study was conducted among instructors in faculty of Nursing and Midwifery in The-ran. The questionnaire was completed by faculty instructors who were 140 indi-viduals. “Multidimensional Symptoms Fati-gue Inventory-Short Form” was translated then the content validity index, face and concurrent validity were measured. Mea-nwhile, reliability was evaluated by internal consistency and test re-test after two weeks. Findings: The content validity index of “Multidimensional Symptoms Fatigue In-ventory-Short Form”, was 79.2 on relev-ance, 82.5 on clarity and 79.7 on simplicity, respectively. Face validity of the scale was approved according to experts’ opinions. Concurrent validity of “Multidimensional Symptoms Fatigue Inventory-Short Form”, with “Fatigue Severity Inventory” was me-asured and the correlation value was 0.77. Cronbach's alpha of the scale was 0.91 and the reliability was 0.82 through test re-test method. Discussion & Conclusion: The “Multidim-ensional Symptoms Fatigue Inventory-Short Form” is a valid and reliable scale for measuring instructors’ fatigue in Iran.