Introduction: Diazinon is one of the most important an organophosphate insecticide that widely used for insect control in homes and agriculture. This factor causes induces oxidative stress in different tissues. The aim of this study was to study the protective role of vitamins E and C as antioxidant in reduction of DZN-induced oxidative stress in rat kidney.
 Materials & methods: In present experimental study, male Wistar rats were randomly divided into six groups including: control group received (corn oil as a DZN solvent), DZN group (100 mg/kg), vitamin E group (150 mg/kg), vitamin C group (200 mg/kg), vitamin E+DZN group and vitamin C+DZN group, intraperitoneally. 24 hours after injection, rats were anesthetized by ether and kidneys were removed quickly. After tissues hemogenation, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) anzymes activities and glutathione (GSH) and malondialdehyde (MDA) concentrations were determined by biochemical methods.
 Findings: DZN increased SOD, CAT and GST activities in kidneys. Also, reduced GSH concentration and increased concentrations of MDA were observed. Administration of vitamins E and C prevent changes in these parameters in the kidneys.
 Discussion & Conclusion: DZN by increasing lipid peroxidation membrane and reduced GSH concentration cause oxidative stress in kidneys. Administration of vitamins E and C as an antioxidant decrease DZN toxicity via purging the free radicals but not completely.