Materials and Methods: All 160 clinical samples have been taken from various samples of hospitalized patients after diagnosis of E. coli, antibiotic resistance was surveyed by Kirby-Bauer method. For resistant isolates, double disk phenotypic confirmatory test was carried out in order to diagnosis ESBL-producing strains. Then from ESBL-producing strains, DNA extracted And bla_TEM genes were detected using PCR. 

Finding :Among 160 clinical isolates of E. coli which were collected, the maximum resistance to Amoxicillin has been observed in all strains, and all were susceptible to Imipenem, and 51.9% of strains were ESBL positive, that 27 strains (32.5%) of bla_TEM genes were observed using PCR.

 Discussion &Conclusions :In this study, more than 50% were detected as ESBL-producing strains, which More than half of the isolates were bla_TEM positive. Comparing these results with other studies in this field shows a direct relations between development of resistance to beta-lactam antibiotics and genes transfer such as bla _TEM.