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:: Volume 21, Issue 2 (6-2013) ::
Journal of Ilam University of Medical Sciences 2013, 21(2): 170-176 Back to browse issues page
The Evaluation of Ethanol Effects on Fibroblast Cells Viability
P Amini gram , S Kashl kashl , M Zamanian azodi , M Rezaee tavirani , Gh Basati , M Rezaei tavirani * 1
1- , rezaei.tavirani@ibb.ut.ac.ir
Abstract:   (16818 Views)
Introduction: Ethanol known as ethyl alc-ohol is a volatile, flammable, colorless liq-uid. It is primarily known as the type of alcohol found in alcoholic beverages. Its consumption is very high around the world. It has been reported that ethanol intake is associated with different diseases. There-fore, here in this study the effect of ethanol on human fibroblast cells was investigated. Materials & Methods: We have carried out cell survival and morphologic studies via Invert Microscope and MTT assay methods to evaluate survival rate and morphological alterations of human fibroblast cells treated with different concentrations of alcohol. Findings: Our findings suggested that etha-nol could possibly change human fibroblast cells morphology and survival rate after 48 h incubation. In addition to this, examining fibroblast cells after 48 h culturing without ethanol showed the maintenance of these changes in next generation of the cells. Discussion & Conclusion: It can be conclu-ded that ethanol can possibly cause genetic alterations of the human fibroblast cells, so karyotype evaluations is required to deter-mine these consequences.
Keywords: ethanol, fibroblast, MTT assay, morphologic studies
Full-Text [PDF 458 kb]   (2556 Downloads)    
Type of Study: Research | Subject: Microbiology
Received: 2013/08/6 | Accepted: 2013/09/11 | Published: 2013/09/11
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amini gram P, kashl kashl S, zamanian azodi M, rezaee tavirani M, basati G, rezaei tavirani M. The Evaluation of Ethanol Effects on Fibroblast Cells Viability. J. Ilam Uni. Med. Sci. 2013; 21 (2) :170-176
URL: http://sjimu.medilam.ac.ir/article-1-1129-en.html

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Volume 21, Issue 2 (6-2013) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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