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Isolation and characterization of selenite resistant microorganisms from industrial wastewaters
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Mohammadreza Zolfaghari *   1, Mohaddeseh Khalilian2 |
1- islamic Azad University , mreza.zolfaghary@gmail.com
2- islamic Azad University |
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Abstract: (8367 Views) |
| Introduction: High concentrations of soluble selenium, selenite and selenate are toxic to living organisms. The accumulation of selenium in certain environments results in discoloration of the skin, hair loss, deformation of nails, tooth decay, weakness and a lack of mental alertness, liver and kidney necrosis and cell death. Thus, bioremediation has been considered as an effective means of cleaning up of selenium-contaminated sites.
Materials & Methods: In this study, 263 strains were isolated from wastewater samples collected from selenium-contaminated sites in Qom Province using the enrichment culture technique and direct plating on agar. MIC was measured by agar dilution method in resistant strains and the disk diffusion method was performed to determine resistance or sensitivity for these bacteria.
Findings: Bacterial strains designated QW90 and QW243 exhibited very high MIC values ranging 550 and 500 for toxic oxyanion of selenite, respectively. Strains QW90 and QW243 identified as Bacillus sp. AB315f (FRB21125) and Pseudomonas marincola (AF326382) with conventional biochemical tests and 16S rRNA studies. These two bacterial strains were resistant to some antibiotics.
Discussion & Conclusion: Our study showed utilization of enrichment culture technique in comparing to the direct plating on agar lead to better isolation of selenite resistant bacteria. Also, Bacillus sp. strain QW90 could be further used for bioremediation of contaminated sites. |
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| Keywords: MIC, selenite, resistance, bioremediation |
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Full-Text [PDF 569 kb]
(3438 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2013/07/15 | Accepted: 2013/12/1 | Published: 2014/02/9
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