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:: Volume 33, Issue 4 (9-2025) ::
Journal of Ilam University of Medical Sciences 2025, 33(4): 130-146 Back to browse issues page
Determining the agent dominant species of human and animal Brucellosis in endemic areas of Ilam province
Mehdi Shokri1 , Razi Naserif2 , Shahab Falahi2 , Saeed Khoshnood3 , Ali Jalilian4 , Ebrahim Babaahmadi5 , Somaye Chamanara6 , Hossein Ebrahimpour6 , Heshmatola Alirahmi6 , Jalal Naseri6 , Elahe Alivaysi7 , Fariba Shokri *8 , Morteza Shams2
1- Dept of Internal Medicine, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
2- Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran
3- Clinical Microbiolog Research Center, Ilam University of Medical Sciences, Ilam, Iran
4- Dept of Vector biology and control diseases, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran
5- Dept of pathobiology, Faculty of Para-Veterinary Medicine, Ilam University, Ilam, Iran
6- Central Labotatory of Veterinary Medicine of Ilam Province, Ilam, Iran
7- Dept of Clinical Biochemistry, Ilam University of Medical Sciences, Ilam, Iran
8- Tuberculosis and Lung Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran & Dept of Internal Medicine, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran , drfaribashokri@gmail.com
Abstract:   (46 Views)
Introduction:  Brucellosis is one of the most important zoonotic diseases in Iran and the world. The aim of this study was to determine the predominant species of human and animal brucellosis in Holeylan County, one of the endemic areas of the disease in Ilam Province.
Materials & Methods: In this cross-sectional study from April to September 2024, a total of 1000 samples of raw and unpasteurized bulk milk from 50 cows, 500 sheep, and 450 goats were collected from traditional domestic farming in Piaz Abad village, Holeylan county. In this research, 10 blood samples from suspected cattle, 63 blood samples from suspected sheep, 43 blood samples from suspected goats, and 23 blood samples from humans were collected. Samples were screened by slide agglutination and ring tests. The seropositive samples were examined by a collection of specific primers for Brucella abortus and Brucella melitensis using PCR tests in SPSS V.22 at a significant level of 0.01.
Results: Results showed that 140 sheep, 60 goats, and 1 cattle sample were positive in the milk ring test. A total of 23 sheep samples, 10 goats, and 8 humans were positive in the slide agglutination test. No positive samples were found in the cattle. A similar band of 905 bp was observed for all samples. 7 sheep samples (30.43%), 2 goats (20%), and 1 human (12.5%) were positive in PCR. In sequencing, the dominant animal species was Brucella melitensis.
Conclusion: The dominant animal species in endemic areas of Ilam province was Brucella melitensis. This study has health and environmental significance because the disease has an animal origin and contaminates the environment around humans.


 
Keywords: Malt Fever, Holeylan, Endemic, Brucella abortus, Brucella melitensis, PCR
Full-Text [PDF 1192 kb]   (26 Downloads)    
Type of Study: Research | Subject: Bacteriology
Received: 2025/06/3 | Accepted: 2025/07/20 | Published: 2025/09/27
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Shokri M, Naserif R, Falahi S, Khoshnood S, Jalilian A, Babaahmadi E, et al . Determining the agent dominant species of human and animal Brucellosis in endemic areas of Ilam province. J. Ilam Uni. Med. Sci. 2025; 33 (4) :130-146
URL: http://sjimu.medilam.ac.ir/article-1-8655-en.html
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Volume 33, Issue 4 (9-2025) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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