[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 2 (6-2018) ::
sjimu 2018, 26(2): 133-140 Back to browse issues page
Identification and Investigation of norA Gene Mutations by Nucleotide Sequencing in Ciprofloxacin Resistance Staphylococcus aureus Isolated from Nasal Infection in Sanandaj Town, Western Iran
Sheida sadat Zonoori 1, Sabriyeh Amini 2
1- Young Researchers and Elite Club, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , sheidazonoori@gmail.com
2- Dept of Cellular and Molecular Biology, Islamic Azad University, Sanandaj, Iran
Abstract:   (424 Views)

Introducrtion:Introduction: Fluoroquinolones are among useful antibiotics against infections caused by Staphylococcus aureus, but today resistance to these classes of antibiotics is a serious problem in patient’s treatments. Overexpression of norA gene is a Fluoroquinolone resistance mechanism in Staphylococcus aureus to be studied through our investigation.
 
Materials & Methods: In this study, 50 Staphylococcus aureus strains were isolated from nasal infection in Sanandaj town. These strains were then identified by conventional biochemical tests. Disk diffusion testing for antibiotic resistance assessment and PCR for existence of norA gene were done. Then, PCR products were sent to Bioneer Company for sequential testing. As a result, nucleotides and amino acid mutations were studied, based on sequencing results and Expasy translator.
 
Findings: The results of biochemical tests were as coagulase, mannitol fermentation, DNase and hemolysis positive, novobiocin sensitive and polymyxin resistance. Oxacillin (42%), erythromycin (46%), doxycyclin (10%), amikacin (20%), tetracycline (24%), penicillin (68%), ciprofloxacin (10%), vancomycin (6%), nalidixic acid (70%) were the antibiotics to which resistance rate was assessed. All the resistance strains had norA gene and 4 identical points of mutation were identified as, G491->A, C537-> T, C593-> C and A585->G respectively. Then, after translation in D315 base, aspartic acid (D) had been converted into Glycine (G).


Discussion & Conclusion:  According to our results, common nucleotide and amino acid mutations were observed in four strains of ciprofloxacin resistant Staphylococcus aureus. Therefore, more studies are needed to find out if the mentioned mutation is the cause of overexpression of norA.

Keywords: staphylococcus aureus, disk diffusion, blast, expasy, sequencing
Full-Text [PDF 599 kb]   (110 Downloads)    
Type of Study: Research | Subject: Medical microbiology
Received: 2016/11/1 | Accepted: 2017/03/5
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

zonoori S S, Amini S. Identification and Investigation of norA Gene Mutations by Nucleotide Sequencing in Ciprofloxacin Resistance Staphylococcus aureus Isolated from Nasal Infection in Sanandaj Town, Western Iran . sjimu. 2018; 26 (2) :133-140
URL: http://sjimu.medilam.ac.ir/article-1-3913-en.html


Volume 26, Issue 2 (6-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
Persian site map - English site map - Created in 0.23 seconds with 31 queries by YEKTAWEB 3767