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Showing 8 results for Colorectal Cancer
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Volume 16, Issue 1 (4-2008)
Abstract
Abstract
Introduction: The incidence of colorectal cancer has increased in Iran in recent years and become the fourth most common cancer in both sexes. The aim of this study was to define the prognostic factors in Iranian colorectal cancer patients using univariate and multivariate methods.
Materials & methods: All patients with colorectal carcinoma admitted to the Cancer Registry Center of Shahid Beheshti university of medical sciences during Jan 1, 2002 to Jan 1, 2007 were analyzed. Survival rates of all the cases were determined. The probability curves for survival were calculated according to the Kaplan–Meier Method, then compared by the Log-rank test. Multivariate analysis was carried out using the Cox proportional hazard model.
Findings: Out of 1138 cases, a survival information was obtained on 1127 patients, 690 males (61.2%) and 437 females (38.8%). The mean survival time was 105.1(CI: 95,1-115.1) months. The Kaplan-Meier method indicated that, the 1, 3, 5, 7, 10 and 15 years survival rates were 91.1, 73.1, 61.0, 54.9, 47.9, 25 and 9 percent, respectively. These variables were independent prognostic factors for survival as determined by multivariate analysis, that is, tumor size, metastasis of tumor, body mass index, marital status, and grade of tumor.
Conclusion: Our results showed that stage of tumor, state of metastasis, grade of tumor and tumor size should be considered the most important prognostic factors in colorectal cancer patients. There fore, early detection of cancer using screening programs can improve survival in such patients.
Ms Zahra Akbari, Dr Seyed Reza Mohebbi, Dr Mahdi Montazer Haghighi, Mr Mohammad Yaghoob Taleghani, Dr Mohammad Amin Pour Hossein Gholi, Mr Seyed Mohammad Hossein Kashfi, Ms Mahsa Khan Yaghma, Mr Behzad Damavand, Professor Mohammad Reza Zali,
Volume 21, Issue 5 (10-2013)
Abstract
Background & Aim:
Colorectal cancer (CRC) is the third common type of cancer and the second leading cause of death in cancer. DNA damages and chromosomal instability are the greatest risk factors favoring the development of CRC. Exo1 is the only exonuclease involved in the MMR system. In this study because of the important role of Exo1 and in order to assess the susceptible biomarkers associated with the CRC , we have investigated the correlation between SNP of Exo1 gene rs1047840 and risk of CRC.
Material & Method:
This case-control study was performed on 118 cases and 130 healthy controls who had been referred to Taleghani hospital of Tehran. We analysed genotyping using polymerase chain reaction- restriction fragment length polymorphism and using MseI restriction enzyme.
Results:
According to our findings the frequencies of AA, AG,GG genotypes in control group were 49/3%, 43/8% and 6/9% and in patient group were 44/9%, 47/5% and 7/6% . The frequency of G and A allels were 71/2% and 28/8% in healthy controls and 68/6% and 31/4% in patients.
Conclusions:
Base on our findings, rs1047840 polymorphism is not associated with susceptibility to CRC and so we reached on a conclusion that this polymorphism possibly doesn’t have significant role in increasing or decreasing risk of CRC.
A Baghestani, M Gohari, A Oroji, Ma Pour Hosaingholi,
Volume 22, Issue 3 (7-2014)
Abstract
Estimation of Prediction Error for Survival Time with Application in Colorectal Cancer
Baghestani A1, Gohari M2, Orooji A1*, Pourhosseingholi A3
(Recived: July 20, 2013 Accepted: March 9, 2014)
Abstract
Introduction: Colorectal cancer is one of the most widespread and killer among canc-ers and it is highly important to predict the survival time. The purpose of this paper is fitting the Cox model and Kaplan-Meier cu-rve for the colorectal cancers data in Tale-ghani Hospital and comparing both with apparent loss.
Materials & Methods: This paper is related to colorectal cancer patients in Tehran Tal-eghani Hospital who were followed up for at least five years. These patients referred to Taleghani Hospital from 2001 to 2006 and their information was recorded in the cancer section of Digestion Research Center and was followed up for at least five years. The Cox Proportional Hazards model and Ka-plan- Meier curve were used for prediction of survival times and these were compared with estimation of prediction error by apparent loss method.
Findings: Cox model showed a significant relation between size of tumor and sex with survival time. The prediction error with ap-parent loss method for Cox model was at times less than Kaplan-Meier curve.
Discussion & Conclusion: In this study, we concluded that estimation of prediction error could be used for comparison of mo-dels
Pariya Azarng, Changiz Ahmadizadh,
Volume 27, Issue 3 (8-2019)
Abstract
Introduction: The imbalance of the microbial ecosystem causes biological changes associated with colorectal cancer and cell proliferation and their planned death, as well as the immune responses. The aim of this study was to determine the effect of probiotic bacteria on the expression of genes associated with growth, metastasis and apoptosis in cancer cells of the HT29 colon.
Materials & Methods: During this study, probiotic Acetobacter were cultured and treated with HT29 cancer cells after preparation of media. The MTTassay method was investigated. DNA extraction from the treated cells was performed and the DNA Ladder assay test was performed. After extraction of RNA and preparation of cDNA, the expression of genes related to growth (AKT1 and PTEN) and apoptosis pathway gene (BAX) was measured by Real-timePCR.
Findings: The results of the MTT assay showed that the Acetobacter bacteria inhibited the proliferation of HT-29 cells and induced apoptosis in these cells. The DNA ladder assay results from treatment of HT29 cells with these bacteria showed qualitative changes in cell apoptosis. In addition, the results of Real-time PCR indicated that the bacterial strains increased the expression of BAX (PTEN) genes in the cancer cells of the HT29 colon.
Discussion & Conclusions: Acetobacter stimulates cellular apoptosis signaling pathway in cancer cells of the HT29 colon and it can be used as an adjuvant therapy in colon cancer treatment or in new treatment strategies.
Arezo Zarei, Saeid Ghorbian,
Volume 29, Issue 3 (8-2021)
Abstract
Introduction: There is growing evidence about the use of antioxidants to reduce the side effects of chemotherapy and cancer drug resistance. Therefore, this study aimed to use vitamin C as an antioxidant and determine its effect on drug resistance in HT29 cells.
Materials & Methods: During this case-control study, HT29 cells were first cultured and evaluated by MTT assay for cell death in the presence of vitamin C and 5FU. DAPI staining was also performed to visualize cell apoptosis. After RNA extraction and cDNA preparation, to determine the molecular mechanism of apoptosis by the drug and the inhibition effect or aggravation of vitamin C on cellular signaling of apoptosis, expression of growth-related genes and apoptosis of Caspase-3 and Bax were measured by real-time PCR.
Findings: The results of the MTT test showed that vitamin C had no significant effect on cell apoptosis induced by 5-FU. DAPI and DNA ladder assay results from HT29 cells showed qualitative changes in cell apoptosis. In addition, real-time PCR results revealed increased expression of Bax and Caspase-3 in HT29 colon cancer cells treated with chemotherapy.
Discussions & Conclusions: Our findings showed that vitamin C did not prevent the effect of chemo drug although it had no positive effect on the growth of cancer cells. Therefore, vitamin C intake by patients undergoing chemotherapy will not affect the drug's effect on the tumor and may protect the patient from side effects.
Musa Yaghoubizadeh, Leila Pishkar, Gholam Basati,
Volume 30, Issue 3 (8-2022)
Abstract
Introduction: Studies have shown that an increase in carcinoembryonic antigen (CEA) is associated with the progression of colorectal cancer and is considered a sensitive diagnostic factor for CRC. Moreover, the role of peroxisome proliferators (PPARs) has recently been considered in colorectal cancer. This study aimed to investigate the relationship between the expression level of PPARs and CEA level in patients with colorectal cancer.
Material & Methods: In this study, a total of 100 samples of primary tumor tissue along with adjacent healthy tissue samples and serum samples of patients with colorectal cancer who underwent surgery were prepared from the tumor bank of Tehran Cancer Institute in Thran, Iran. The expression level of peroxisome proliferator receptors (PRARs) in tissue samples and CEA level in serum samples were measured, and the relationship between these markers was evaluated as well. The financial support for the study was provided by the Islamic Azad University (grant number 132655).
(Ethic code: 132655)
Findings: The results of this study showed a significant increase in the expression level of PPARs and serum CEA levels in patients with colorectal cancer (P<0.01). Although the level of these markers had a significant relationship with the progression and spread of disease in patients (P<0.01), no significant relationship was observed between the expression of PPARs and serum CEA in these patients (P>0.05).
Discussion & Conclusion: Based on the obtained results, the expression level of PPARs and the serum CEA level are associated with the progression and spread of the disease; however, there is no significant relationship between the expression level of PPARs and the serum CEA level in patients with colorectal cancer.
Sanaz Salek, Elham Moazamian, Afshin Mohammadi Bardbori Mohammadi Bardbori, Seyedeh Azra Shamsdin,
Volume 32, Issue 6 (2-2025)
Abstract
Introduction: The efficacy of 5-fluorouracil (5-FU) as a chemotherapy drug for colorectal cancer (CRC) is well-established. However, due to resistance to 5-FU and its associated complications, it is necessary to search for adjuvant therapies against CRC. Considering the anticancer potential of probiotic metabolites, this study assessed the anticancer effects of potential probiotic strains isolated from mule milk, camel milk, and the standard probiotic strain Lactobacillus rhamnosus GG (LGG), used alone and in combination with 5-FU, against the human colon cancer cell line (HT-29) and normal human embryonic kidney (HEK-293).
Materials & Methods: Biochemical and molecular techniques were implemented to identify the strains isolated from mule and camel milk. The MTT assay was also employed to assess the anticancer effects of the probiotic strains of Lactobacillus isolated from mule milk and camel milk and of LGG, used alone and in combination with 5-FU, on the two cell lines HT-29 and HEK-293. The data were statistically analyzed using analysis of variance, and significant differences between means were assessed using Tukey's post hoc test. Minitab 20 software performed all statistical calculations in the level of significance less than 0.05.
Results: The results demonstrated the potential probiotic properties of all the studied Lactobacillus strains. The combination of cell extracts from Lactobacillus strains with 5-FU effectively reduced the viability of HT-29 cells. Nonetheless, this combination can reduce the cytotoxic effects of 5-FU on the viability of HEK-293 cells, thereby increasing their viability.
Conclusion: These results point to the possibility of using Lactobacillus strain cell extracts as an adjuvant therapy for cancer treatment
Zahra Salimizadeh, Sattar Tahmasebi Enferadi, Tayebeh Majidizadeh, Razieh Jalal, Frouzandeh Mahjoubi ,
Volume 33, Issue 1 (4-2025)
Abstract
Introduction: Colorectal cancer is a prevalent digestive tract malignancy, with oxaliplatin being an effective chemotherapeutic drug, but long-term use can lead to drug resistance. It is crucial to develop methods to reduce CRC cells' oxaliplatin resistance. Peganum harmala L. (P. harmala), is a common anticancer treatment in traditional medicine.
Materials & Methods: The study used P. harmala seeds extract to treat oxaliplatin-resistant HCT116 cells for 24 and 48 hours. Cell viability was determined using the MTT assay, and RNA extraction was performed from the treated and control cells. cDNA synthesis was performed using extracted RNAs, and the expression level of the BAX gene was analyzed using Real-Time PCR. Statistical analysis was conducted using Graph Pad Prism 8 software and t-test, with a significance level of P less than 0.05.
Results: Findings indicated a significant correlation between the increase in the concentration of extract and the percentage of dead cells. The cytotoxicity of the aqueous extract of P. harmala seeds significantly increased from 24 to 48 hours in oxaliplatin-resistant HCT116 cells. Furthermore, a significant and considerable upregulation in the relative expression of the BAX gene was observed compared to the control group (P<0.001).
Conclusion: The cytotoxic effect of the aqueous extract of P. harmala is dose- and time-dependent. The extract has the potential to induce apoptosis in colorectal cancer cells by regulating apoptosis-related genes, particularly by upregulating pro-apoptotic genes such as BAX.
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