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Showing 2 results for Spermatogonial Stem Cells
Emad Reza, Hossein Azizi, Ali Asghar Ahmadi,
Volume 31, Issue 1 (4-2023)
Abstract
Introduction: One of the vital enzymes during spermatogenesis, which is one of the pluripotency factors of stem cells and contributes to maintaining their pluripotency is alkaline phosphatase. ZBTB16 and ZFP proteins are critical elements in stem cells which are expressed in pluripotent stem cells and maintain their pluripotency due to their role in messaging pathways.
Material & Methods: The separation of the spermatogonial stem cells is the initial stage, followed by the preparation of the mouse embryonic stem cells and ES-like cells. The protein-protein network and the connections between genes were then investigated after alkaline phosphatase staining. Then, using the Fluidigm PCR method, the expression of the ZBTB16 and ZFP genes was determined. Finally, spermatogonia was immunohistochemically stained for the ZBTB16 protein.
(Ethic Code: IR.AUSMT.REC.1400.29)
Findings: Alkaline phosphatase was expressed positively by stem cell types; moreover, it was absent in Sertoli and fibroblast cells. Additionally, the specificity of these factors was demonstrated by the positive expression of the ZBTB16 and ZFP genes in these three cells and their negative expression in Sertoli cells. The connection and effect of these genes on the other genes are shown by the Protein-Protein network analysis.
Discussion & Conclusion: Alkaline phosphatase, as well as ZBTB16 and ZFP genes are expected to be among the special markers of pluripotent stem cells and the critical elements of embryonic stem-like cells.
Maedeh Yazdani Dizicheh , Hossein Azizi , Dariush Gholami , Amir Khaki ,
Volume 32, Issue 4 (9-2024)
Abstract
Introduction: Spermatogenesis is the primary process of sperm production occuring in seminiferous tubules. Spermatogonial stem cells (SSCs) have the ability for self-renewal, differentiation, and the transmission of genetic information to subsequent generations. KLF4 and POU5F1 are transcription factors expressed in a wide range of tissues and play key roles in such processes as apoptosis, differentiation, proliferation, and cellular development. The present study aimed to assess the expression levels of KLF4 and POU5F1 genes in mouse embryonic stem cells (mESCs), spermatogonial stem cells (SSCs), embryonic-like stem cells (ES-like), and testicular cells, and identify the signaling pathways associated with them in the process of spermatogenesis.
Materials & Methods: In this experimental study, spermatogonial cells were extracted from mouse testes using enzymatic digestion method and cultured in GSC medium containing FGF, EGF, and GDNF. Thereafter, the expression of KLF4 and POU5F1 genes was examined in mESCs, SSCs, ES-like, and testicular cells was investigated using immunocytochemistry, immunohistochemistry, and reverse transcription-polymerase chain reaction methods, and protein-protein interactions and signaling pathways were evaluated using bioinformatics methods.
Results: The KLF4 and POU5F1 genes exhibited positive expression in ES-like cells and testicular cells. The assessment of KLF4 mRNA and POU5F1 mRNA expression levels demonstrated that KLF4 expression is higher in mESCs and ES-like cells compared to other cells, while POU5F1 expression is higher in SSCs. Both KLF4 and POU5F1 are considered essential and powerful genes that share a common class and function.
Conclusion: The findings of this study indicated that KLF4 and POU5F1 play crucial roles in the proper development of sperm and are present in various types of cells, including mESCs, SSCs, ES-like cells, and testicular cells. These factors are key components of sexual stem cells and contribute to stem cell proliferation, making them potential diagnostic markers for these cell lines.
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