Introduction: Herbal plants have been used for the treatment of different abnormalities such as spasm disorders. Some of these plants have gastro intestinal property. One of these herbs in iranian traditional medicine is Thymus migricus which used for antispasmodic activity and its effects present in essential oil. The present study was designed to find out the effects of Thymus migricusessence on mechanical activity of the isolated rat’s ileum.

Materials & methods: In this study, for evaluation of spasmolythic property of  essense, different doses of the solution were added to organ bath after acetylcholine (Ach) with concentration of 10-4 molar (M) and for assessment of its antispasmodic, different doses of the solution were added to the organ bath before Ach with concentration of 10-6upto 10-3 M, then isotonic contraction of  ileum were recorded in three groups of control, test and shamthrough an isolated tissue chamber in an organ bath by using isotonic transducer and oscillographic device.

Findings:The  results from  different concentration of Thymus migricusessence  on Ach 10-4 M  showed that concentration of 50 ng/ml  of essense and higher was reduced to 95% of Ach induced contraction which is significant (P< 0.05).

Discussion & Conclusions: Thymus migricusessence hasspasmolythic and antispasmodic property.these effects may be produced by Thymol which is present in its essence and reduced calcium activity and release by sarcoplasmic reticulum.