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Showing 2 results for Pou5f1
Hossein Azizi, Amirreza Niazi Tabar, Atiyeh Mohammadi,
Volume 28, Issue 5 (11-2020)
Abstract
Introduction: Spermatogonial Stem Cells (SSC) are the originators and beginning points of the spermatogenesis process. Moreover, they are considered the only stem cells in the body that could transfer genetic information to the next generation through gametogenesis. This study aimed to investigate the potency and power of SSC under in vitro and in vivo conditions.
Materials & Methods: Enzymatic digestion technique was utilized to extract the spermatogonial cells of the pig and mouse's testis. They were then cultured in an environment containing FGF, EGF, GDNF, and a feeder layer of STO. For immunocytochemistry and RT-PCR analysis, Ki67, POU5F1, and ZBTB16 markers were used to evaluate the resulted colonies. Ethics code: Ir.ausmt.rec.1398.03.07
Findings: The nature of the SSC resulted after separation and culture was proved through measures, such as cluster growth of the colonies in the culture medium, Ki67 marker expression in the immunocytochemistry review which showed the duplication ability, and the morphological criteria observed by an electron microscope. Moreover, the comparative expression of POU5F1 and ZBTB16 markers in the embryonic stem cells, SSC, and Sertoli cells within the seminiferous tubules of the mouse was analyzed by RT-PCR.
Discussions & Conclusions: This experimental study investigated the expression of Ki67, POU5F1, and ZBTB16 in the seminiferous tubules and special cytological features of SCC. The findings are beneficial for future advanced studies in reproductive biology fields.
Maedeh Yazdani Dizicheh , Hossein Azizi , Dariush Gholami , Amir Khaki ,
Volume 32, Issue 4 (9-2024)
Abstract
Introduction: Spermatogenesis is the primary process of sperm production occuring in seminiferous tubules. Spermatogonial stem cells (SSCs) have the ability for self-renewal, differentiation, and the transmission of genetic information to subsequent generations. KLF4 and POU5F1 are transcription factors expressed in a wide range of tissues and play key roles in such processes as apoptosis, differentiation, proliferation, and cellular development. The present study aimed to assess the expression levels of KLF4 and POU5F1 genes in mouse embryonic stem cells (mESCs), spermatogonial stem cells (SSCs), embryonic-like stem cells (ES-like), and testicular cells, and identify the signaling pathways associated with them in the process of spermatogenesis.
Materials & Methods: In this experimental study, spermatogonial cells were extracted from mouse testes using enzymatic digestion method and cultured in GSC medium containing FGF, EGF, and GDNF. Thereafter, the expression of KLF4 and POU5F1 genes was examined in mESCs, SSCs, ES-like, and testicular cells was investigated using immunocytochemistry, immunohistochemistry, and reverse transcription-polymerase chain reaction methods, and protein-protein interactions and signaling pathways were evaluated using bioinformatics methods.
Results: The KLF4 and POU5F1 genes exhibited positive expression in ES-like cells and testicular cells. The assessment of KLF4 mRNA and POU5F1 mRNA expression levels demonstrated that KLF4 expression is higher in mESCs and ES-like cells compared to other cells, while POU5F1 expression is higher in SSCs. Both KLF4 and POU5F1 are considered essential and powerful genes that share a common class and function.
Conclusion: The findings of this study indicated that KLF4 and POU5F1 play crucial roles in the proper development of sperm and are present in various types of cells, including mESCs, SSCs, ES-like cells, and testicular cells. These factors are key components of sexual stem cells and contribute to stem cell proliferation, making them potential diagnostic markers for these cell lines.
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