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Showing 2 results for Fgf
Somayeh Moghaddam, Esmaeil Babaei,
Volume 30, Issue 1 (3-2022)
Abstract
Introduction: Long non-coding RNAs (lncRNAs) and miRNAs belong to a class of non-coding RNAs (ncRNAs) that play important roles and functions in the regulation of the expression of genes in main biological processes, such as cell proliferation, apoptosis, and differentiation. LncRNAs can potentially affect miRNAs in the forms of cis/trans to modulate their regulatory role. In this study, mRNA, miRNA, and lncRNA gene networks were predicted by web-based programs for three ectodermal pathway markers (BMP4, NOG, FGF8) in the mouse embryonic stem cells.
Material & Methods: In this theoretical bioinformatics study, the miRNAs of the target genes (BMP4, NOG, and FGF8) were extracted and examined by MirWalk and TARGETSCAN databases to finally obtain the common miRNAs of these three genes. Following that, the target lncRNAs for common miRNAs were then extracted from the DIANA-Tool database.
(Ethic code: 100/21560/2/پ)
Findings: MiRs mmu-miR-92a-2-5p, mmu-miR-129b-5p, mmu-miR-130b-5p, mmu-miR-692, mmu-miR-7009-3P, mmu-miR-7116-3p, and mmu-miR-7689-3p may affect the function of lncRNAs, including Kcnq1ot1, Gm26812, Gm4117, Gm11837, 4930423MO2Rik, Malat1, Gm12594, Gm3414, 5830444B04Rik, Gm2464, and NEAT1.
Discussion & Conclusion: Due to the mutual relationships among lncRNA, miRNA, and mRNA, our results provided a novel perspective on lncRNAs for future research and experimental studies on ectodermal differentiation pathways and molecular mechanisms.
Mohammadreza Pourmohammad Pourmohammad, Jina Khayatzadeh , Seyyedeh Hananeh Akbari , Sima Afsharanjad , Saeedeh Zafar Balanjad,
Volume 32, Issue 5 (11-2024)
Abstract
Introduction: Angiogenesis is the biological process of sprouting new vessels from existing vessels in the tissue. The main factors in the molecular guidance of this process are vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Given the significance of antibiotics in reducing angiogenesis through the inhibition of collagenase, phospholipase A2, and several matrix metalloproteinases, the objective of this study was to examine the impact of the Doxycycline antibiotic on alterations in the gene expression of VEGF and FGF within the angiogenesis pathway of the chorioallantoic membrane (CAM) in chick embryos.
Materials & Methods: In this research, 50 Ross fertilized eggs were randomly divided into 5 groups, including the control group, laboratory control, and 3 experimental groups. On the eighth day of incubation, the laboratory control group was treated with normal saline, and the experimental groups were treated with doses of 50, 100, and 250 µmol/ml of the Doxycycline antibiotic. On the twelfth day, photographs were taken of the samples. The number and length of blood vessels and then the weight and length of embryos were measured. A real-time PCR technique was used to investigate the expression changes of VEGF and FGF genes on the CAM membrane. The statistical tests included one-way ANOVA, independent t-test, and post hoc tukey, which were analyzed by SPSS V.20 at a level of significance less than 0.05.
Results: The number of vessels and the length of vessels in the treatment groups showed a significant decrease compared to the control group (P<0.05). Also, the expression level of VEGF and FGF genes in the treatment groups was significantly lower than the control group (P<0.05).
Conclusion: The doxycycline antibiotic can significantly reduce the expression level of VEGF and FGF genes and the angiogenic process of the chorioallantoic membrane of chick embryos.
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