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Showing 2 results for marandi
Maryam Banparvari, Khalil Khayambashi, Seyed Mohammad Marandi, Seysd Jamal Moshtagian, Abas Salehikiya,
Volume 23, Issue 7 (2-2016)
Abstract
Introduction: Osteoporosis is defined as a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissue, resulting in increased risk of fracture. However, appropriate exercise for the prevention and treatment of osteoporosis has not been determined. The aim of this study was to determine the effects of two progressive loading training program on the bone properties of Osteoporotic rat models.
Materials & methods: Among 30 male rats, 6 rats were separated as healthy group, Then Osteoporosis was induced in remaining rats by intraperitoneal injection of 20% ethanol solution (3g/kg/day) for four consecutive days for 3 weeks. 24 Osteoporotic rats were divided into 4 groups (n=6): baseline (As the baseline of the experimental groups), resistance training, endurance training and control. The two training groups completed 12 weeks of training program, 5 days a week according to protocol. The Endurance protocol includes running exercise on a treadmill for, 12 m/min, 10 to up 64 min/ day. The Resistance protocol consisted of 8 series of climbs on the 110-cm vertical ladder angled at 80º with weight tied to animal tail that was increased from the 50% of the body mass of the animal in the first series to 100% in the eighth series. At the end of the intervention, the animals were euthanized and the Bone mineral density (BMD) of the femur and the L4 +L5 were measured using dual energy x-ray absorptiometry (DXA). Microarchitectures of the proximal tibia metaphysis tissue were measured by using standard histomorphometric methods and with the motic software.
Findings: The endurance (p= 0.035) and resistance (p= 0.001) groups had significantly increased femur BMD compared to controls. L4+L5 BMD in resistance training and control was significantly greater than endurance group (p= 0.001,p= 0.001). only the resistance training had significantly increased the trabeculare percent (p= 0.018) and cortical thickness (p= 0.009) and had significantly decreased trabecular separation percent (p= 0.02)compared to controls. A number of osteocyte in both endurance and resistance groups was significantly greater than that in control group (p= 0.03, p= 0.002).
Discussion & Conclusions: Resistance training induces more effective favourable changes in bone mineral status and pathology as compared to endurance training in osteoporotic male rats.
Sayyed Gholamhoseyn Rahimi, Sayyed Mohammad Marandi, Milad Abdollahi, Samaneh Shirkhani, Mohammad Hossein Nasr-Esfahani,
Volume 31, Issue 4 (9-2023)
Abstract
Introduction: The increasing prevalence of obesity has affected millions of people across the globe, leading to various diseases, such as prediabetes, diabetes, cardiovascular diseases, cancer, and muscle disorders. One of the therapeutic interventions in the prevention of diabetes is the use of herbal supplements, such as green coffee and its phenolic compounds, including chlorogenic acid, due to its antioxidant, anti-inflammatory, and insulin-sensitive properties. Therefore, the present study aimed to investigate the effect of green coffee and chlorogenic acid on glycogen content in the muscles of prediabetic mice.
Material & Methods: A total of 20 male C57BL/6 mice were randomly assigned to two groups: normal diet mice (5 mice) and high-fat diet mice (15 mice), which were fed for 12 weeks. After the induction of prediabetes by Fasting blood sugar (FBS) and Glucose tolerance test (GTT) tests, this group was divided into three subgroups: prediabetes mice (5 cases), prediabetes mice with diabetes with green coffee consumption (Prediabetes - Green Coffee, 5 cases) and prediabetes with chlorogenic acid consumption (Prediabetes - Chlorogenic Acid, 5 cases) which were treated for 10 weeks. In the end, the mice were sacrificed, and the relative level of expression of genes involved in the glycogen content of the Gastrocnemius muscle, including PI3K, AKT, GSK3, GYS1, as well as the LRRC8A gene, was measured through the Real-Time PCR technique. The data were analyzed using the one-way analysis of variance test by GraphPad Prism software (version 8).
Findings: The results pointed to a significant increase in the blood glucose level in the HFD group (129.9±5.4 mg/dL) compared to ND (95±3.3 mg/dL) and a significant decrease in the PD-GC group (103.5±8.16 mg/dL) and PD-CA (99±8 mg/dL) compared to the PD group (129.5±9.4 mg/dL) (P<0.001). The periodic acid-Schiff (PAS) staining technique demonstrated a significant decrease in the level of glycogen content in the rats of the PD group compared to the ND group. While in the treatment groups, a significant increase was observed in muscle glycogen accumulation compared to the PD group (P<0.001). The expression level of PI3K, AKT, LRRC8A, and GYS1 genes decreased, and GSK3 significantly increased in the PD group compared to the ND group (P<0.001), indicating a disturbance in the signaling pathway of glycogen content in the PD group. Nonetheless, in the treated groups, a significant increase was observed in the expression level of PI3K, AKT, LRRC8A, and GYS1 genes compared to the PD group (P<0.001).
Discussion & Conclusion: Considering the complications caused by prediabetes disease, in addition to early diagnosis of prediabetes, it seems to control weight and increase insulin sensitivity, the consumption of green coffee and chlorogenic acid can improve the prediabetic condition caused by the signaling pathway of glycogen synthesis in the muscle tissue of prediabetic rats.
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