---

Introduction: Sirtuin-3 (SIRT₃) acetylates and activates the mitochondrial enzymes and helps to preserve mitochondrial integrity and prevents apoptosis through regulating Oxo-Guanine Glycosylase-1 (OGG-₁). Resveratrol could stimulate SIRT₃ and the athletes use this supplement to support the effects of training. This study aimed to evaluate the effect of continuous training and Resveratrol consumption on SIRT₃ and OGG₁ proteins in the liver tissue of male rats.
 
Materials & Methods: In total, 32 adult male rats were divided randomly into four equal groups of control (1. Con), Resveratrol supplement consumption (2.R), Continuous exercise training (3.C), and Continuous exercise+Resveratrol (4.C+R). Continuous exercise training was performed for 6 weeks (6 days per week) at 80% of VO₂max on a treadmill.  In addition, groups 2 and 4 received 50 mg/kg body mass/day of Resveratrol. At the end of the protocol, the rats were anesthetized with   Ketamine/Xylazine, and their liver samples were removed to evaluate the protein levels of SIRT₃ and OGG₁ using an enzyme linked immunosorbent assay. The data were analyzed through a one-way Anova test. A pvalue less than 0.05 were considered statistically significant.
 
Findings: The results indicated that Resveratrol consumption was significantly increased the SIRT₃ levels in the second group (i.e., R) (P=0.01) and the fourth group (i.e., C+R) (P=0.0001); moreover, it led to an increase in the OGG₁ level in the fourth group (i.e., C+R) (P=0.0001); however, it had no effects on the OGG₁ levels in the R group (P=0.91). In addition, SIRT₃ and OGG₁ levels were significantly increased in the third group (i.e., C) (P=0.0001), compared to the R group.
 
Discussion & Conclusions: A combination of Resveratrol supplement with continuous exercise training would have probably more positive effects on SIRT₃ and OGG₁ levels, compared to the training or the consumption of the supplement alone; therefore, this supplement is recommended to be used with continuous exercises.