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Showing 2 results for Nasrolahi omran
Salman Ahmadyasbchin, Ayatollah Nasrolahi Omran, Arman Rostamzad, Naser Jafari,
Volume 21, Issue 7 (2-2014)
Abstract
The heavy metal pollution has become one of the most important environmental problems. Heavy metals are the most important environmental pollutants, especially for human health and aquatic systems are a serious threat. Iranian Research Organization for Science and Technology of yeast Saccharomyces carlsbergensis PTCC 5051, and received as a lyophilized culture YEDPA environment and then the proliferation of malt extract broth is used. The effects of pH, temperature, kinetics and isotherm of lead on metal uptake were evaluated by the SC. Absorption maximum at about pH 5 the optimum temperature was 25 degrees Celsius. A kinetic study showed that batch biosorption of lead to rapid removal by the biomass of Saccharomyces was the first test was done in less than 30 minutes. Method using FT-IR, surface functional groups of fungi were identified. With active and passive absorption of lead by Saccharomyces has revealed more attracted by the yeast is active. With yeast and control yeast on the autoclave, the pre-treated with sodium azide and 2 and 4 DNP nitro phenol showed that claims of capturing, respectively, 0/68, 0/12, 0/44 and 0/48 mmol g was obtained. Results showed that, even using the passive yeast are suitable for the absorption of lead . Keywords: Biosorption, lead, Saccharomyces carlsbergensis, 2, 4-Dinitrophenol, Sodium azide
Niusha Kaveh, Ayatollah Nasrolahi Omran, Mohammad Taghi Hedatyati, Mojtaba Taghizade Armaki, Mahdi Abastabara, Saeed Mahdavi Omran6,
Volume 32, Issue 2 (6-2024)
Abstract
Introduction: Candidiasis is a fungal infection caused by Candida yeasts in immunocompromised patients. This study aimed to assess antifungal susceptibility of clinical isolates of Candida species to ketoconazole and nystatin by broth microdilution.
Material & Methods: In this descriptive-analytical study, 377 clinical isolates of Candida species were collected from different regions of Iran. The species were identified using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method. The antifungal susceptibility testing of clinical isolates of Candida species to ketoconazole and nystatin was performed using broth microdilution according to standard protocol CLSI (M27-A3 and M27-S4). The standard strain of Candida parapsilosis was used as the quality of control. The minimum inhibitory of concentrations was determined after 24h at 37 ºC.
Results: Based on molecular identification, Candida albicans 266 (70.7%), C. krusei 64 (17%), C. parapsilosis 40 (10.7%), and C. tropicalis 6 (1.6%) were identified. The MIC90 and MIC50 values of ketoconazole and nystatin for all isolates were 0.063 µg/ml, 0.031 µg/ml, 2 µg/ml, and 4 µg/ml, respectively.
Discussion & Conclusion: As evidenced by the results of this study, antifungal susceptibility testing along with the identification of fungi at the species level would certainly be very useful in selecting primary antifungal agents for treatment, especially in invasive fungal infections. According to our findings, ketoconazole was much more effective on Candida spp. than nystatin.
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