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Showing 12 results for Kiani
S Samadi, M Kiani Zadeh , M Fathi Najafi , S D Mousavi Nasab, A M Hossein Nia Davatgar , M Jafari, N Ahmadi, R Azizian,
Volume 21, Issue 1 (5-2013)
Abstract
Introduction: Newcastle disease is an extr-emely contagious disease that infects many species of birds. Newcastle Disease Virus (NDV) belongs to the paramyxoviridae fa-mily and genus Rubulavirus. The mem-brane of the virus particle contains HN and F glycoproteins. Cleavage of the F glycop-rotein has a direct relation with the patog-enicity of the virus. The aim of this study was to determine the gene and protein sequences of F glycoprotein in virus strains isolated from recent outbreaks in Iran.
Materials & Methods: Six NDV Viruses were passaged and their RNA isolated. Using RT- PCR technique, cDNA prepar-ation and amplification of the gene were done. Isolates were assessed by electropho-resis and observed with UV. The isolates were digested with restriction enzyme Pst I. Finally, in order to corroborate the ampl-ified PCR product, DNA of isolates were purified, dried and sequenced.
Finding: In this study all of the NDV strains contained a 935 bp band that after digestion with Pst I, produce two bonds 720 bp and 180 bp. Nucleotide analysis showed single base mutations but that was not significant amino acid sequence and anal-ysis indicated no amino acid change in the cleavage site of F protein.
Discussion & Conclusions: Absence of point mutations in the cleavage site of F protein illustrated the importance of the maintained cleavage site of protein F among the strains studied.
Gh Kiani Feizabadi, A H Mahvi, M H Dehghani,
Volume 23, Issue 4 (10-2015)
Abstract
Introduction: The main concern in composting plant is leachate generation that is known to be highly polluted wastewater. Migrations of untreated leachates are potential sources of hazardous contaminants in soil, groundwater and surface water. This study was aimed to investigate the heavy metals removal efficiency from Isfahan composting leachate using the following coagulantsferrous sulfate, ferric chloride, poly ferric sulfate, alum and poly aluminum chloride.
Materials & methods: This is an experimental study. Leachate samples were collected from the Isfahan composting leachate´s collection ponds. At first, leachate characteristics including COD, BOD, TSS and pH as well as the following heavy metals Zn,Cu,Ni ,Cr, Cd were measured according to the standard methods. Jar-test experiments were carried out to examine the effects of changing coagulants' dosage (0.5, 1, 1.5, 2, 2.5 and 3 g/l) and pH values (4-12) on heavy metal removal. As a result, the effective dosage, optimum pH and the most convenient coagulant were identified.
Findings: investigating the average of mentioned heavy metals' concentration, Zn had the highest concentration in leachate (6.2mg/l). The optimum pH for precipitation of the metals using alum, polyaluminiumchloride, ferricchloride, ferrous sulfate and poly ferric sulfate was 6.5,7,10,10 and 11 respectively. Optimum concentration of the mentioned coagulants was obtained 2, 1.5, 1.5, 2 and 2g/l respectively. Poly ferric sulfate with70% to 87% of heavy metals removal and 50% of COD removal had the highest efficiency. Poly aluminiumchloride with 65% to 85%, Ferric chloride with 75% to 80%, ferrous sulfate with70% to 80% and finally alum with 70% to75% were the following priorities at heavy metals removal.
Discussion & Conclusion: Poly ferric sulfate is the most effective coagulant to remove heavy metals from the leachate and comparing the results with the Iranian guidelinefor effluent discharge shows that the concentration of heavy metals in leachate did not exceed the maximum allowed values.
Zahra Mousavi Pourgohar, Dr Davoud Kianifard, Dr Emad Khalilzadeh,
Volume 24, Issue 4 (11-2016)
Abstract
Introduction: Testicular tissue is susceptible to environmental risk factors. Structural and functional alterations in spermatogenesis are the most important factors involved in fertility complications. Methylphenidate is amphetamine derivatives. This drug can approve for treatment of attention-deficit/hyperactivity disorder (ADHD), tachycardia syndrome, narcolepsy, depression. There is a little data about the effects of methylphenidate on testicular tissue. In this study, time dependent effects of methylphenidate were evaluated on testicular tissue in microscopic and ultrastructural levels.
Materials & methods: 30 adult Wistar rats were divided into three groups as control, short-term methylphenidate (10 mg/kg/day) treated for two weeks and long-term methylphenidate (10 mg/kg/day) treated for eight weeks. Histomorphological and ultrastructural assessments were prepared on testicular tissue through routine histologic and electron microscopic methods.
Findings: Some alterations such as tubular atrophy and transformation and disarrangement of spermatogenic cells were observed in testicular tissue following time dependent administration of methylphenidate. Deformation of mitochondria, increment of collagen fibers and basement membrane thickness, were the main cellular and structural alterations observed in ultrastructural level.
Discussion & conclusions: The results of this study indicated that the process of spermatogenesis reduces temporary following short term administration of methylphenidate; however, it seems that repeated administration of methylphenidate progressively leads to some degrees of tissue habitation and resistance.
Jhila Hamidi, Davoud Kianifard, Gholamreza Vafaei Saiah, Reza Hazrati,
Volume 24, Issue 5 (12-2016)
Abstract
Introduction: Monosodium glutamate is a food additive which acts as preservative or enhancer of palatability. Some studies show some adverse effects of this agent on reproductive system like as structural and functional alterations and reduction of fertility. According to various antioxidant properties of quince leaves and the cytotoxic effects of monosodium glutamate, the aim of this study was to evaluate the protective effects of quince leaf extract on testicular tissue alterations induced by monosodium glutamate.
Materials & methods: 60 adult rats were divided into six groups: 1) control; 2) monosodium glutamate (30mg/kg i.p.); 3) monosodium glutamate (60mg/kg i.p.); 4) monosodium glutamate (30mg/kg i.p.) + quince leaf extract (500mg/kg p.o.); 5) monosodium glutamate (60mg/kg i.p.) + quince leaf extract (500mg/kg p.o.) and 6) control + quince leaf extract (500mg/kg p.o.). At the end of eight weeks, histomorphometeric and spermatogenic evaluations were done on testicular samples.
Findings: The results showed that, administration of monosodium glutamate lead to structural and functional alteration of testicular tissue such as tubular atrophy and spermatogenic amendments while, use of quince leaf extract can reduce the revealed alterations.
Discussion & conclusions: According to the findings, it is concluded that antioxidant herbs such as quince leaf, can be effective in reducing of structural alterations of testicular tissue induced by monosodium glutamate.
Ghasem Akbari, Amirali Shahbazfar, Davoud Kianifard, Heresh Rezaei, Siamak Shokrollahi, Mrs Donya Mohebi,
Volume 24, Issue 6 (2-2017)
Abstract
Introduction: Burning injuries are among physical and chemical destructive phenomena which sometimes cause death . Anti bacterial effects of olive oil and lime water has been proved previously. Olive oil and lime water are used to treat burning injuries in Iran by traditional practitioners . The aim of this study was a microscopic survey on the healing effects of olive oil and lime water on second degree burning injuries in rat .
Material & methods: In this study, 36 Wistar rats were used. After the production of a second degree burning injury, the rats were randomly divided into three groups. The first group were treated with the combination of olive oil and lime water, the second group were treated with silver sulfadiazine 1% and the third group received normal saline. Untill the end of the 4th week 3 of the rats were killed each week and tissue specimens were taken from them. Afterwards microscopic slides were made of them. The slides were studied qualitatively using a light microscope. The number of blood vessels were counted in the surface unit too.
Findings: The number of blood vessels enhanced with the time. This enhancement is more in the group of olive oil and lime water. With the maturity of the wounds the number of the vessels reduced. Qualitative study showed that olive oil and lime water treat the injuries faster and vascularization, granulation tissue growth and maturity and epithelialization were faster in this group.
Discussion & conclusions: Olive oil and lime water can be used as a cheap treatment for second burning injuries.
Azad Abdollahzadeh, Davoud Kianifard, Gholamreza Vafaei Saiah, Javad Ashrafi Helan,
Volume 26, Issue 3 (9-2018)
Abstract
Introduction: Methylphenidate (MPH) is one of the common medications used for maintaining alertness and improving attention. Monosodium glutamate (MSG) is a food additive, which acts as an enhancer of palatability. The aim of this study was to evaluate the various effects of these compounds on the reproductive system during adolescence.
Materials and Methods: Methylphenidate (5 and 10 mg/kg) and monosodium glutamate (6 and 60 mg/kg) were administrated to adolescent rats. After 60 days, the pituitary-testicular axis hormones were assayed and testicular histomorphometric studies were performed.
Findings: The coadministration of MPH (5 mg/kg) and MSG (60 mg/kg) led to elevation in serum FSH levels (P<0.05). The highest blood level of luteinizing hormone was observed following high doses of MPH and MSG separately or in combination form. The effect of MPH and MSG on serum testosterone level was observed dose dependently and contradictorily (P>0.05). The administration of MSG led to a reduction in the population of Sertoli cells, spermatogonia, and spermatocyte. Coadministration of MPH and MSG also reduced this population. Morphometric studies revealed decrement of tubular diameter and germinal epithelium height (P<0.05), especially with low doses of MPH and high doses of MSG. The changes in spermatogenic indices were similar to morphometric results. Tubular atrophy, interstitial edema, and depopulation of spermatogenic cells were observed in MPH and MSG treated groups. Coadministration of these compounds increased spermatids with pyknotic nucleus.
Conclusion: It has been concluded that the coadministration of MPH and MSG through the induction of some hormonal and structural alterations could induce some changes in the normal structure and function of the reproductive system.
Ayoob Sabaghi, Ali Heyrani, Amir Kiani, Namdar Yousofvand,
Volume 27, Issue 1 (4-2019)
Abstract
Introduction: Depression is one of the most important psychological disorders in people with epilepsy. However, the effect of seizure on depression during pregnancy has not been studied experimentally. Therefore, the purpose of this study was to examine the effect of Seizure on depression in pregnant mice under treatment with pentylenetetrazol (PTZ).
Materials & Methods: After pregnancy stabilization in mice, one group was classified as the naive group. the rest of the kindled mice were assigned into three groups including: seizure induction group between the 14th and 19th days of pregnancy once in 48 hours, the sham group received normal saline equal to PTZ intraperitoneally on the 14th and 19th days of pregnancy once in 48 hours and the control group which consisted of pregnant mice kindled without injection during pregnancy. The rate of depression in pregnant mice was measured between 14th and 19th days of pregnancy once in 48 hours and two h after PTZ injection using Forced Swim Test.
Findings: The results showed that depression in PTZ-treated pregnant mice was significantly higher than in other groups; moreover, the difference was statistically significant (P<0.05).
Discussion & Conclusions: According to the obtained results, seizure in pregnancy results in the increase of drpression levels consequently, it is necessary to identify patients in order to prevent them from depression and therefore avoid negative effects of depression on offspring.
Leila Kiani, Amin Rafeipoor, Maryam Mashayekh, Ramin Tajbakhsh, Jaffar Pouyamanesh,
Volume 27, Issue 3 (8-2019)
Abstract
Zahra Ramezani, Hasan Tahermansouri, Farhoush Kiani,
Volume 29, Issue 5 (12-2021)
Abstract
Introduction: The most common ocular tumor is retinoblastoma, which can be treated by different methods. The use of carbon nanostructures has attracted much attention due to their unique properties, such as crossing cell membranes, for cancer diagnosis and other medical applications. This study aimed to investigate and assess the toxicity of the modified graphene oxide (GO) with isatin-3-semicarbazone (ISA) in order to treat retinoblastoma.
Material & Methods: This study utilized ISA to modify the GO. Subsequently, the modified GO-ISA was characterized by different techniques, such as Fourier transform infrared spectrometry, Scanning Electron Microscope, and Thermal Gravimetric Analysis. The cell toxicity of samples was assessed by MTT assay for two types of cells of retinoblastoma (Y79) and adipose-derived stem cells (ADSC) after 24 h at different concentrations. Data were analyzed in SPSS software using one-way analysis of variance (ANOVA). A p-value less than 0.05 was considered statistically significant.
Findings: The results showed that in the concentration range of 10-50 µL, GO-ISA decreased significantly (P<0.01) the conservation percentage of retinoblastoma cells from 73.98% to 27.48%, while these values for ADSC decreased from 78.16% to 50.11% (P<0.01). In addition, GO-ISA at 30 µL resulted in a significant increase (P<0.01) in the cell toxicity of the retinoblastoma (26%), compared to GO.
Discussion & Conclusion: GO-ISA increases the toxicity of the retinoblastoma cells, compared to GO which was significant at 30 µL. Therefore, this modification of the surface can be useful in future applications. With this modification of the surface, the active sites are provided for the biological and pharmacogenetic reactions in future.
Khatoon Sedaghat, Marzieh Mahmoodi, Jamileh Kiani, Seyed Homamodin Javadzade, Mahnoush Reisi,
Volume 31, Issue 3 (8-2023)
Abstract
Introduction: Healthcare workers, who work at the frontline of providing health services, are at risk of occupational stress due to several conditions. Therefore, the present study was conducted to determine the effects of virtual training based on Bandura's self-efficacy theory on occupational stress of healthcare workers in Bushehr, Iran.
Material & Methods: This semi-experimental study was conducted on 63 healthcare workers in Bushehr city in 10 comprehensive healthcare centers randomly selected as intervention and control centers (n=5 each). The intervention group received four sessions of virtual training based on self-efficacy theory in the form of a webinar, while the control group received no training. The information of the studied subjects was collected using a questionnaire consisting of four sections, namely demographic information assessment, self-efficacy assessment, and occupational stress assessment before, two weeks, and one month after the educational intervention. The data were analyzed using SPSS software version 24.
Findings: In general, knowledge and self-efficacy increased in the intervention group after training, and changes in knowledge and self-efficacy were significantly different between the two groups during the study period (P<0.001). In the intervention group, occupational stress improved two weeks and one month after training, compared to the pre-training period, whereas in the control group, although the changes were significant, these changes manifested as an increase in stress intensity. There was a significant difference in occupational stress changes between the two groups during the study period (P<0.001), and the condition improved in the intervention group.
Discussion & Conclusion: The results of this study suggested that virtual stress management education based on self-efficacy theory could increase knowledge levels and self-efficacy and improve occupational stress in healthcare workers.
Zahra Mohammadi Kiani, Ali Mahdad,
Volume 31, Issue 6 (2-2024)
Abstract
Introduction: Feeling alone and enduring distress are risk factors for suicidal thoughts, while parental bonds are protective factors. However, the complex mechanisms behind these factors have not been identified among people with a history of suicide attempts. Therefore, the present study aimed to predict suicide attempts through distress tolerance, feeling alone, and parental bonds in people with a history of suicide attempts.
Material & Methods: The current research is a descriptive-correlational study, and the statistical population included all people with a history of suicide attempts in Isfahan, Iran, from among whom 120 people were selected through convenience sampling. The research tools included parental bond questionnaires by Parker et al. (1979), distress tolerance by Simmons and Gaher (2005), feelings of loneliness by Russell et al. (1980), and attempted suicide was made by the researcher. The collected data was analyzed using descriptive methods, including Mean and Standard deviation and inferential statistics (e.g., Pearson's correlation coefficient and stepwise regression analysis) using the SPSS software (version 27) and PLS3 statistical software.
Results: The results showed that among the predictor variables, feeling alone had the highest predictive power (64.7%), and in the second step, with the addition of parental bond with the mother, it increased to 0.701%, and in the third step, with the addition of Parenting bond with father reached 0.729%, and in fourth step with the addition of distress tolerance, it reached 0.743%, all of which are statistically significant.
Discussion & Conclusion: Based on the findings of the present research, we can point out the influential role of feeling alone in predicting suicide attempts, and the need for families and policy-makers to pay attention to this prominent factor can play a major role in reducing suicide rate.
Shabnam Tofighi, Seyedeh Arezoo Hosseini, Parinaz Hamed Nasimi, Mahsa Kiani,
Volume 32, Issue 6 (2-2025)
Abstract
Introduction: Hysterectomy is one of the most common surgeries among women, and it may be associated with complications such as depression and sexual dysfunction post-surgery. However, the results of existing studies are controversial. This study aimed to investigate the levels of depression and sexual function following hysterectomy in women who visited Ayatollah Mousavi Hospital in Zanjan (Iran) from 2018 to 2020.
Materials & Methods: This retrospective cohort study was conducted on 63 married women who underwent hysterectomy surgery. Depression levels were assessed using the Beck Depression Inventory (BDI), and sexual function was evaluated with the Female Sexual Function Index (FSFI). The data were statistically analyzed using SPSS V.24.
Results: The average depression score of patients was 8.91±10.58, and their sexual function score was 22.18±18.29. 85.8% of individuals had mild depression, and 65.1% experienced sexual dysfunction. Among the sexual function subscales, pain during intercourse was reported by 60.3% and vaginal dryness by 50.8% of patients. 81% expressed satisfaction with their bodies, while 19% were dissatisfied due to the absence of the uterus.
Conclusion: Depression severity after at least one-year post-hysterectomy falls within the mild to moderate range, and body image is generally positive in many cases. However, the results regarding sexual function indicate dysfunction, with pain during intercourse and vaginal dryness being the most common complaints. Addressing these sexual function issues through education, follow-up, and treatment can improve patients’ sexual well-being after surgery.
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