Introduction: class B beta-lactamases such as IMP and VIM that read metallo-β-lactamase(MBL), due to the hydrolysis of penicillins, cephalosporins and carbapenems for the teatment of infectious diseases, major difficulties create. In study, the prevalence of MBL blaIMP and blaVIM genes in urinary isolates of Pseudomonas aeruginosa were investigated in ilam province.
Materials & methods: A total of 60 strains of P. aeruginosa Central Laboratory of ilam, were collected and were identified using biochemical methods Antibiotic susceptibility testing by the disk diffusion method(Kirby-Bauer) forantibiotics cefepiem, ceftriaxone, Aztreonam, gentamicin, amikacin, ciprofloxacin, ofloxacin, ceftazidime, piperacillin tazobactam and imipenem was performed. Then strain insensitive to ceftazidime, the method Ceftazidime - EDTA Combined disk synergy test(CDST-CAZ) Was used to detect MBL-producing strains. The PCR test strains using specific primers for blaIMP and blaVIM genes was investigated.
Findings: A total of 17 strains were resistant to ceftazidime. With method CDST all strains non-susceptible to ceftazidime, were MBL-producers. PCR and sequencing methods proved that 6 isolates were positive for blaVIM genes, whereas none were positive for blaIMP genes. All isolates that were positive for VIM gene were resistant to all antibiotics studied except piperacillin tazobactam.
 Discussion & conclusion :In this study, blaVIM dominant gene in P. aeruginosa urinary strains resistant to ceftazidime was administrative. Given the importance of MBL-producing strains in hospitals, rapid detection of these strains could be crucial step in the treatment and control of infections caused by these strains is considered.

Introduction: Osteoarthritis is the most common joint disease in humans. Footprints inflammation in osteoarthritis is not a new discovery. The degenerative change in inflammatory joints is associated with overproduction of inflammatory cytokines. In the process, the monocyte and macrophage cells enter the synovium and play a role in the production of the cytokines. The aim of this study was to investigate the effect of the essential oil of Nigella sativa L in reducing expression of pre-inflammatory cytokines in human macrophage and monocyte cells in comparison with steroid anti- inflammatory drugs in a model similar to osteoarthritis.
 
Materials & methods: Culturing THP-1 cells, providing Nigella sativa L essential oil, determining the degree of toxicity through SDH, LDH and MTT methods, treatment of the cells with LPS, placebos and essential oil, isolation of RNA and then, producing cDNA were performed. The expression of TNF-α and IL-18 was determined by RT-PCR method and analyzed through ANOVA, F static, and REST methods.
 
Findings: The essential oil of Nigella sativa L reduced the expression of TNF-α and IL-18 and has a significant difference (p<0.05) with the control group.
 
Discussion & conclusions: The results showed that the Nigella sativa L essential oil reduces the expression TNF-α and IL-18 in THP-1 cells stimulated with LPS and can be used in treating inflammatory diseases due to its anti- inflammatory properties.