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Showing 3 results for Farahmand
Farzaneh Farzanfar, Saide Parvaresh, Zahra Farahmandinia, Afshin Sarafinejad, Navid Gharaei,
Volume 25, Issue 3 (9-2017)
Abstract
Introduction: Infectious complications in pediatric oncology patients are a major cause of morbidity and mortality. Early detection of bacterial infection and prescription of antibiotics prevented from complication such as sepsis and death. The present study aimed to evaluate the diagnostic value of IL8 compared with ESR and CRP in the detection of bacterial infection is done.
Materials & methods: In this study , 48 children with cancer (18 were females and 30 males with a mean age 57.17 months) with high grade fever 38.5 in 2014 referring to Afzalipur hospital in Kerman, were examine. Plasma was obtained from pediatric oncology patients at the reception time with febrile neutropenia and 48 h later. Blood samples were taken for testing IL8 , centrifuged and the serum was isolated. The samples were frozen at -70 over a period of 13 months and samples of the Keith Human IL-8 / NAP-1 Platinum made in USA were measured using an ELISA assay. The patients were classified as having or not having a bacterial infection. Serum levels of IL8, ESR, CRP were measured in both groups and Independent sample T-test and SPSS version 22 were used for statistical analysis.
Findings: From 48 children, 18 (37.5%) were females and 30 (62.5%) were males. Overall mean age was 57.17 months. From 48 patients , 9 cases had bacterial infection including 4 cases bacteremia, 3 cases pneumonia and 2 cases UTI and others had non-bacteria. Data showed that the highest amount of sensitivity and specificity marker belonging to IL8 is 100, the lowest amount of sensitivity attribute ESR marker is 66.7 and the lowest amount of specificity attribute CRP marker is 84.6.
Discussion & conclusions: IL-8 is the most useful marker for the early detection of bacterial infections , compared with CRP , ESR and this marker can be a useful tool for evaluating patients who are at the high risk of infection and candidate for intravenous antibiotics.
Zolfaghar Lotfi, Fatemeh Maleki, Somayeh Farahmand,
Volume 31, Issue 5 (11-2023)
Abstract
Introduction: The extract of Thymus kotschyanus Boiss, in addition to its antioxidant properties, also has a marvellous anti-amyloidogenic effectthat can be used for the treatment of amyloidosis. The aim of this study was to prepare silver nanoparticles from the aqueous extract of mountain thyme (Thymus kotschyanus Boiss), to investigate its antioxidant and inhibitory effect on the production of amyloid nanofibrils (bovine serum album (BSA) as a model protein).
Material & Methods: This study was conducted experimentally in the laboratory of Payam Noor University in Qom Province in 2022. First, nanoparticles were prepared from aqueous thyme extract and added to AgNo3 silver nitrate solution (one millimolar). ThenFTIR, EDX, DLSand SEM methods were used to study the properties and size of the nanoparticles. The removing of free radicals 2,2-diphenyl-1-picrylhydrazyl (DPPH) method was used to study the antioxidant effects of the extract and the Concorde absorption method was used to study the production of amyloid nanofibrils. The chemical composition of the extract was analysed using the gas chromatography method. For statistical analysis, descriptive statistics and ANOVA tests were used with SPSS software version 23.
Findings: The phytochemical analysis of the extract revealed seven compounds. Most compounds were associated with 5,4-alpha-epoxy-3-methoxy (43.53%), 2-ethyl-piperidine-1-methyl (31.02%) and thymol (23.65%). The UV-Vis spectrum showed the highest absorbance at 460 nm. The SEM results confirmed the production of spherical nanoparticles with a size of 70-93 nm. The concentrations of 0.8 and 1 mg/ml of the extract were able to inhibit DPPH by more than 50%. The highest antioxidant activity in the sample containing 2 ml of the nanoparticle solution (sample 5) was 56.82%. The highest inhibition of amyloid nanofibril production was observed at a concentration of 2 mg/ml with 95%.
Discussion & Conclusion: Thus, the synthesized nanoparticles have potent antioxidant and anti-amyloidogenic properties and can be used as antioxidants.
Somayeh Farahmand , Farshid Rezaei , Iraj Alipourfard ,
Volume 33, Issue 1 (3-2025)
Abstract
Introduction: Due to the increasing resistance of bacterial diseases to antibiotics, it is essential to identify medicinal plants that contain antibiotics and antibacterial. This study aimed to compare the efficacy of pasture thyme essential oil with common antibiotics against Escherichia coli and Staphylococcus aureus bacteria.
Materials & Methods: The study, conducted in 2022 at Payame Noor University, investigated the antibacterial properties of Thymus eriocalyx essential oil. Leaves and stems were collected from Kermanshah highlands, and essential oil was extracted using hydrodistillation. Gas chromatography-mass spectrometry identified the components. The oil was tested against Escherichia coli and Staphylococcus aureus at concentrations of 0.48 to 250 µg/mL using liquid and solid methods, as well as the disk diffusion method. Results were compared with antibiotics (ampicillin, ciprofloxacin, erythromycin, and cefixime) at specific concentrations. Data were analyzed using SPSS V.23 with a significance level of 0.01.
Results: Tests with gas chromatography-mass spectrometry showed that carvacrol (66.96%) is the main ingredient in this type of thyme, and a total of 42 active substances were found. Essential oil concentrations of 250, 125, and 62.5 mg/ml were more effective than the chosen antibiotics in inhibiting both bacterial strains. The minimum bactericidal concentration for Escherichia coli and Staphylococcus aureus was determined to be 62.5 and 7.81 mg/ml, respectively.
Conclusion: The essential oil of Thymus eriocalyx demonstrated greater effectiveness against Staphylococcus aureus than Escherichia coli. Due to its significant antibacterial and antiseptic properties compared to antibiotics, further studies on its efficacy in animal models are recommended.
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