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Showing 8 results for Azizi Jalilian
R Khalesi, J Salimian, Sm Moazeni, F Azizi Jalilian,
Volume 20, Issue 4 (2-2013)
Abstract
Enterotoxigenic Escherichia coli is the most common bacterial agent producing diarrhea in the world wide. This bacterium includes some virulence factors such as colonization factors and heat-labile toxin and/ or heat-stable toxin. Enterotoxigenic Escherichia coli attached to the intestinal epithelial cell surface through CFs and causes diarrhea by toxin secretion. Molecular pathogenesis stu-dies demonstrate that there are many pro-phylactic approaches for this infection. The best possible way is designing and prod-ucing efficient vaccine that enabeling to induc protective immunity against the most of bacterial strains. Vaccine candidate molecule(s) that are used to design a vaccine, in addition to possess immuno-genicity and safety they should be wid-espread among variety of strains and co-ntain conserved and common epitops. This article pays attention to review some infor-mation and results of conducted researches on enterotoxigenic Escherichia coli vaccine and importance of the bacteria and its pathogenesis.
R Azizian, F Azizi Jalilian, H Askari, A Naser, S Karimi, N Sadeghifard, Sd Mosavi Nasab, N Ahmadi,
Volume 20, Issue 5 (3-2013)
Abstract
Background: Pseudomonas aeruginosa is a ubiquitous organism which has emerged as a major threat in the hospital environment. Overuse of antibiotics has also significantly increased the emergence of antimicrobial multiresistant bacteria. P. aeruginosa has an innate ability to adhere to surfaces and form virulent biofilms. Bacteriophage might represent one attractive solution to this problem. In this study, P.aeruginosaphage was utilized to Biofilm prevention and removal.
Methods: After isolation of phages and serial dilution microplate method of biofilm formation was examined. Furthermore, the effects on biofilm bacteria isolated phages were determined on the Pseudomonas putida, Acinetobacter baumanii and E.coli.
Result: P.aeruginosa biofilm had OD: 1.688 in 492n.m. Pure phage, 10-2 and 10-3 diluted phage decreased OD to 1.587, 1.341 and 1.461,respectively.Isolated phage dramatically decline OD of Biofilm of all strains.
Discussion: Phages have various affinity to attach to hosts, thereby it is supposed to phages compete for their receptors.Therefore it is supposed phages have most efficiency in optimum concentration to remove biofilm or growth inhibition. Also, funding prove phages have effect on various bacteria and they can use to remove different strains.
E Rezaee, M Saadati, J Salimian, Gh Ovlad, A Miri, F Azizi Jalilian, Sh Nazarian,
Volume 21, Issue 7 (2-2014)
Abstract
Introduction: Heat labile toxin (LT) is one of the virulence factors of Enterotoxigenic Escherichia coli. B subunit of LT (LHB) is the binding subunit and can induce six months immunity in humans. Tetanus toxin of Clostridium Tetani causes the fatal disease, tetanus. This toxoid induces two years immunity in humans. Hc subunit, as binding domain of the toxoid is considered as immunogenic part of tetanus toxin. The THc and LTB recombinant subunits have the potential role to induce immunological memories with different longevity. The purpose of this study was to evaluate and compare the immunogenicity of THc and LTB recombinant proteins in animal model and also to evaluate their roles in immunity duration.
Materials & methods: The recombinant proteins, THc and LTB, were expressed in the transgenic host, E.Coli Bl21 DE3 using pET28a vector containing their respected genes in optimum condition. After express-ion, LTB and THc were purified from insoluble and soluble phases, respectively. Then, their purity was confirmed by SDS-PAGE gel. To evaluate their immuno-genicity, these proteins were injected into mice and the antibody titer were evaluated and compared by ELISA technique.
Findings: SDS-PAGE results showed overexpressed levels of the proteins under study.Immunity assessment revealed that in the same condition of immunity the THc subunit produces a higher antibody titer in comparison with the LTB subunit.
ِDiscussion & Conclusion: The first step in creating a strong and long-lasting immune-ological memory is the induction of high titer of antibody. Thus, the difference in antibody titer may be related to memory cells lifetime in the immunized mice.
A Amirkhani, M Azizi Jalilian, R Amini, A Amirkhani, K Ashtari, F Azizi Jalilian,
Volume 22, Issue 2 (6-2014)
Abstract
Introduction: Nowadays, excessive pop
ulation growths, urbanization, and cons-umption of fossil fuel lead to the envi-ronmental pollution and damages. So, there is a significant attention to the usi-ng of cleaner fuels such as biogas. Man-y countries use biomass in order to pro-duce biogas as a source of energy. But in Iran, biomass usage has not been yet taken into account. It may be due to the lack ofthe biogas technology and cost. This study aimed to introduce a new ty-pe of semi-automatic green biogas ma-chinewith high efficiencyand enhance its output.
Materials &Methods: In our machine, new mechanical and electronic parts de-vised and economically made. These parts were included: 1- electronic senso-rand monitoringpartfor temperature, hu-
midity and methane variables, 2- seven-layer flexible gas tank, and 3- electronic safety valve with controllable gage for escaping excessive gas.
Findings:The results of the experime-ntal usage of machine showed that the extent of produced gas was acceptable, saf-e, manageable and economic.
Discussion & Conclusion:We could im-prove its efficiency throughcontrolling and changing differentvariablesin trial and error manner. So, useful results ob-tained from this machine showed that this device can be used to enhance the efficiency of methane production by 25 %.
A Naser, F Azizi Jalilian, R Azizian, H Askari, I Pakzad,
Volume 22, Issue 4 (9-2014)
Abstract
Nowadays, excessive use of antibiotics in
the treatment of human and animal diseases
has caused bacteria’s multidrug resistant
rise against antibiotics. Additionally, new
antibiotics’ consumption involves new
resistant genes that can shortly spread
among other bacteria and need new
antibiotics for its control and this requires
spending millions of dollar.Methicillin -
resistant staphylococcus is one of the mos
invasive bacteria that quickly become
resistant to the new generation of
antibiotics. Bacteriophages are viruses that
infect and replicate inside bacteria and
finally lyse the host cells. Phage entry into
the host bacterial cell requires a specific
receptor. Phages are not able to identify
other cells especially eukaryotic cells.
A Amir Khani, M Azizi Jalilian, R Amini , A Amir Khani , K Ashtari, F Azizi Jalilian,
Volume 22, Issue 5 (10-2014)
Abstract
Introduction: Today, we are seeing change in the manufacturing process of mechanis-ms as a multi-purpose device with the dra-matic growth of technology. It both helps in saving space and the cost of the product decreases considerably. Today, due to the cost of providing places for jobs, smaller and more compact multifunctional devices are designed. The proposed device is a du-al-use Laboratory Mixer. This device is designed for mixing liquid with determined viscosity, which has various options to enh-ance the quality and comfort of the operator.Materials & Methods: The device uses a combination of two methods while the process of mixing is separate in other devic-es, i.e. the mixer device is either radial or oscillating. One of the importing points in the design of the device is that both systems are combined in one device and it is equi-pped with control and timing speed system. So far, the roller or Rocker device have not been made and designed in Iran and they are built for the first time. Their main use is for professional mixing experiments in the laboratory of Immunology and medical diagnostics. Findings: The results of the electrical and mechanical performance of the device are acceptable according to the inventions used in various parts and it is economical.Discussion & Conclusion: We could sig-nifycantly reduce blood clots while it is mixing up by designing the angle of the device. This problem is the result of blood into the vial with the vial cap that causes the so-called "lysis". Another achievement of this device is the combining of both the roller and Rocker devices which provides new mixing meth-ods in the lab for operator.
Kobra Heidarzadi, Farid Azizi Jalilian, Ali Reza Rekabi, Razieh Amini, Iraj Pakzad, Morovat Taherikalani, Ali Hematian, Zahra Ghobadian, Maryam Bouchani,
Volume 23, Issue 1 (4-2015)
Abstract
The most common method of oral hygiene is tooth brushing. However, occasionally brushing becomes a risk factor for health. The main reason is getting infected with various microorganisms. The purpose of this study was to investigate bacterial and fungal contamination of toothbrushes students, and factors affecting the contamination.
Materials & Methods: Ninety five brushes were assessed in this study. Samples were incubated in test tubes containing nutrient broth for twenty-four hours. Then the microorganisms were isolated using specific nutrient medium containing Blood agar, Chocolate agar MacConkey and sabouraud dextrose agar. The inoculated plates were incubated for 24 to 48 hours at 37 C. The identification of micro-organisms was performed based on biochemical tests.
Findings: The infection rate was more than 40% of the subject staphylococcus, Niesseria, Diphteroids,Actinomycetes and Enterobacteriacae family members were isolated from samples. Microbial contamination was higher in women than men (P<0.05).There was found correlation between the number of microorganism on brush with time of use, using mouthwash and gender (P<0.05). There was statistically significant relation between cocci microorganisms’ infections with sexes as well as between bacillus infections with capped brush.
Discussion & Conclusion: Based on results, high incidence of bacterial contamination observed in brushes, therefore the proper use, maintenance and timely replacement of toothbrush play an important role in reducing pollution and consequently decreasing oral disease.
Shahab Mahmoudvand, Razieh Amini, Farid Azizi Jalilian, Mojtaba Hedayat Yaghoobi, Masoumeh Javaheri, Iraj Sedighi, Mojgan Mamani, Razieh Ezati, Jalaledin Amiri, Massoud Saidijam,
Volume 30, Issue 3 (8-2022)
Abstract
Introduction: Epidemics and deaths caused by influenza viruses are an important concern worldwide. The use of neuraminidase inhibitors such as oseltamivir is an effective and valuable way to treat the diseases caused by these viruses. However, the mutation in several parts of the gene leads to the emergence of drug-resistant strains, and an ever-increasing rise in drug-resistant strains is a global problem. Histidine-to-tyrosine mutation at position 275 (H275Y) of neuraminidase protein is one of the most common oseltamivir resistance mutations. This study aimed to detect H275Y mutation in influenza A (H1N1) virus circulating in the Hamadan province of Iran using real-time polymerase chain reaction (RT-PCR).
Material & Methods: This cross-sectional study was conducted on 110 swab samples isolated from patients with suspected influenza virus infection between 2015 and 2016. Ribonucleic acid (RNA) was extracted from samples and the RT-PCR method was used to determine virus types and subtypes. The positive samples were evaluated for detection of H275Y mutation using RT-PCR.
(Ethic code: IR.UMSHA.REC.1400.917)
Findings: Out of 110 patients in this study, 50 (45%) were females and 60 (55%) were males. The mean±SD age of participants was 40.74±2.42 years. Influenza A (H1N1) virus was found in 22 (20%) out of 110 patients, including 9/50 (18%) females and 13/60 (21.7%) males. There was no significant relationship between the virus and gender (P=0.81). No drug resistance related to H275Y mutation was observed in 22 positive cases.
Discussion & Conclusion: The findings indicated that no drug resistance mutations have occurred, and oseltamivir is still an appropriate option to treat infections caused by the influenza virus in Hamadan province, Iran. However, due to the increasing number of resistant strains, an annual review of oseltamivir resistance is recommended and further studies are needed in this regard.
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