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Showing 4 results for Abdollahi
A Mahdieh, M Abdollahi, J Hasani,
Volume 22, Issue 4 (9-2014)
Abstract
Cognitive emotion regulation strategies in processing suicidal thoughts
Abstract
Background: Suicide as a major issue is considered in teens and young, because have been reported the second or fourth most common cause of death in this age group. This study examined coping-emotional in suicidal thoughts and attempts.
Method: The research of multi-stage cluster sampling to select the sample was used. All students at would be Khurazmi population. The sample size was 250(n = 101 male students and 149 female students). Instruments included the short form of emotional and cognitive regulation questionnaire and suicidal ideas Questionnaire. The data is performed in order to analyze the results of stepwise, regression is used.
Results: Results show the high correlation of maladaptive cognitive emotion regulation strategies and the idea of suicide ideation. Also compatible strategies for coping with suicidal ideas were correlates and according to awareness of their emotion, this correlation varies.
Conclusions: The findings of this study show that none of the emotional strategies singly anticipate suicidal ideation. But the individual understanding and control of emotions, can predict suicidal ideas. Thus, training programs, educational and emotional healing must pay attention to recognize cognitive emotion to the prevention of suicidal ideas.
Mohammad Ahanjan, Maryam Abdollahi, Behnam Hashemi, Eysa Nazar, Zaher Morsal Jahan,
Volume 26, Issue 5 (1-2019)
Abstract
Introduction: The wideuse of beta-lactam antibacterial agents in the treatment of bacterial infections has increased bacterial resistance. Nowadays, one of the problems in the treatment of nosocomial infections is enzymes resistance to broad spectrum beta-lactamases among clinical isolates, especially Klebsiella pneumoniae.Over the past decade, CTX-M type enzymes have been recognized asthe most common type of broad-spectrum beta-lactamase in Europe, Canada, and Asia.The aim of this study was to evaluate the prevalence of broad-spectrum beta-lactamase-producingKlebsiella pneumoniae bacteria and to identify the CTX-M-3 gene by molecular method.
Materials & Methods: Over a 9-month period, 130 Klebsiella pneumoniaewere isolated from different clinical specimens (urine, blood, ulcers) of infectious patients in Imam Khomeini Hospital, Sari, Iran. Their susceptibility to nine antibiotics used for gram-negative bacteria was measured by disk diffusion agar method. The extended-spectrum beta-lactamases (ESBLs) producing isolates were identified using a hybrid disc method. Moreover, strains producing CTX-M-3 enzyme were detectedby polymerase chain reactionmethod.
Findings: The 48 (36.8%) isolates of Klebsiella pneumoniaewere the producer of ESBLs. Moreover, 22 (16.9%) of these cases contained the CTX-M-3 gene.
Discussion & Conclusions: The obtained results of the currentstudy showed that the prevalence of ESBL producing strains resistant to quinolones is higher in Iran, compared to the developed countries.
Mehdi Masoudi Kerahroudi, Dr Hosein Honari, Seyed Masih E'temad Aubi, Masoud Abdollahi,
Volume 26, Issue 6 (3-2019)
Abstract
- Burkholderia pseudomallei causing Melioidosis and Shigella dysenteriae is the most common cause of diarrhea, and so far no effective vaccine has been produced against these two bacteria. The BLF1 protein of Burkholderia pseudomallei bacteria plays an important role in pathogenesis and infection. Shigella dysentery STxB is one of the most important factors in pathogenesis and also has an adjuvant role. Binding the BLF1 protein with STxB can be a good candidate vaccine. In this study, the antibody titers of BLF1-STxB and BLF1 proteins produced in rats were compared.
Materials & Methods: In this experimental study, pET28a (+) - blf1-stxB and pET28a (+) - blf1 vectors were transformed into E. coli BL21 (DE3) and confirmed by PCR. The expression of blf1-stxB and blf1 genes was induced by IPTG and the proteins were injected into rats four times after purification of the protein by using an affinity chromatography column. Polyclonal antibodies produced in the serum of rats were measured.
- The produced recombinant proteins were approved by SDS-PAGE and Western blotting. ELISA results showed that antibody was produced against the BLF1 antigen and the antibody titer level increased by STxB binding to BLF1, compared with the antibody titer against the BLF1 antigen.
Discussion & Conclusions: The BLF1-STxB protein had higher antibody titer than BLF1. Due to the structural similarity of STxB subunit with its counterpart in E. coli, it can be a vaccine candidate against Burkholderia pseudomallei, Shigella dysenteriae, enterohemorrhagic E. coli, and enterotoxigenic E. coli.
Sayyed Gholamhoseyn Rahimi, Sayyed Mohammad Marandi, Milad Abdollahi, Samaneh Shirkhani, Mohammad Hossein Nasr-Esfahani,
Volume 31, Issue 4 (10-2023)
Abstract
Introduction: The increasing prevalence of obesity has affected millions of people across the globe, leading to various diseases, such as prediabetes, diabetes, cardiovascular diseases, cancer, and muscle disorders. One of the therapeutic interventions in the prevention of diabetes is the use of herbal supplements, such as green coffee and its phenolic compounds, including chlorogenic acid, due to its antioxidant, anti-inflammatory, and insulin-sensitive properties. Therefore, the present study aimed to investigate the effect of green coffee and chlorogenic acid on glycogen content in the muscles of prediabetic mice.
Material & Methods: A total of 20 male C57BL/6 mice were randomly assigned to two groups: normal diet mice (5 mice) and high-fat diet mice (15 mice), which were fed for 12 weeks. After the induction of prediabetes by Fasting blood sugar (FBS) and Glucose tolerance test (GTT) tests, this group was divided into three subgroups: prediabetes mice (5 cases), prediabetes mice with diabetes with green coffee consumption (Prediabetes - Green Coffee, 5 cases) and prediabetes with chlorogenic acid consumption (Prediabetes - Chlorogenic Acid, 5 cases) which were treated for 10 weeks. In the end, the mice were sacrificed, and the relative level of expression of genes involved in the glycogen content of the Gastrocnemius muscle, including PI3K, AKT, GSK3, GYS1, as well as the LRRC8A gene, was measured through the Real-Time PCR technique. The data were analyzed using the one-way analysis of variance test by GraphPad Prism software (version 8).
Findings: The results pointed to a significant increase in the blood glucose level in the HFD group (129.9±5.4 mg/dL) compared to ND (95±3.3 mg/dL) and a significant decrease in the PD-GC group (103.5±8.16 mg/dL) and PD-CA (99±8 mg/dL) compared to the PD group (129.5±9.4 mg/dL) (P<0.001). The periodic acid-Schiff (PAS) staining technique demonstrated a significant decrease in the level of glycogen content in the rats of the PD group compared to the ND group. While in the treatment groups, a significant increase was observed in muscle glycogen accumulation compared to the PD group (P<0.001). The expression level of PI3K, AKT, LRRC8A, and GYS1 genes decreased, and GSK3 significantly increased in the PD group compared to the ND group (P<0.001), indicating a disturbance in the signaling pathway of glycogen content in the PD group. Nonetheless, in the treated groups, a significant increase was observed in the expression level of PI3K, AKT, LRRC8A, and GYS1 genes compared to the PD group (P<0.001).
Discussion & Conclusion: Considering the complications caused by prediabetes disease, in addition to early diagnosis of prediabetes, it seems to control weight and increase insulin sensitivity, the consumption of green coffee and chlorogenic acid can improve the prediabetic condition caused by the signaling pathway of glycogen synthesis in the muscle tissue of prediabetic rats.
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