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:: Volume 34, Issue 3 (7-2026) ::
Journal of Ilam University of Medical Sciences 2026, 34(3): 95-106 Back to browse issues page
The Role of Culture Medium Composition in Regulating Biofilm Formation and Growth of Candida Albicans Cells
Elham Aboualigalehdari1 , Somayeh Mohamadi2 , Maryam Erfaninejad3 , Fatemeh Pourmotahari4 , Mahnaz Fatahinia *5
1- Dept of Parasitology, School of Allied Medical Sciences, Ilam University of Medical sciences, Ilam, Iran
2- Student Research Committee, Ilam University of Medical Sciences, Iran
3- Dept of Basic Medical Sciences, Shoushtar Faculty of Medical Sciences, Shoushtar, Iran
4- Dept of Community Medicine and Public Health, School of Medicine, Dezful University of Medical Sciences, Dezful, Iran
5- Dept of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran & Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , fatahinia@yahoo.com
Abstract:   (125 Views)
Introduction:  Invasive fungal infections, especially with Candida albicans, are a major worldwide health problem. This opportunistic fungus can be pathogenic in immune suppression states or broad-spectrum drug use. It causes both localised and systemic infections. Moreover, C. albicans biofilm formation enhances drug resistance and causes recurrent infections.
Materials & Methods: In this experimental study we compared the adhesion and biofilm formation of C. albicans isolates in Sabouraud Dextrose Broth (SDB) and RPMI-1640 media. Yeast suspensions of standardised concentration were inoculated in flat-bottom 96-well microplates and incubated for 48 h. Biofilm formation was assessed using crystal violet staining and optical density measurements. Data were analyzed using the non-parametric Mann-Whitney test.
Results: Biofilm formation was significantly higher in RPMI-1640 compared to SDB, whereas planktonic growth was more pronounced in SDB.
Conclusion: Our results showed that RPMI-1640 medium with complex nutrients and low glucose concentration was optimal for biofilm formation, whereas SDB with high glucose concentration was better for planktonic growth. The findings underscore the importance of choosing an appropriate culture medium for biofilm studies and could have implications for optimisation of standard treatments against C. albicans infections.
Keywords: Candida albicans, biofilm, culture medium, drug resistance, RPMI-1640
Full-Text [PDF 766 kb]   (47 Downloads)    
Editorial Note: Research | Subject: mycology
Received: 2025/11/14 | Accepted: 2026/05/30 | Published: 2026/07/27
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Aboualigalehdari E, Mohamadi S, Erfaninejad M, Pourmotahari F, Fatahinia M. The Role of Culture Medium Composition in Regulating Biofilm Formation and Growth of Candida Albicans Cells. J. Ilam Uni. Med. Sci. 2026; 34 (3) :95-106
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Volume 34, Issue 3 (7-2026) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
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