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Isolation and determination of Acanthamoeba genotypes in surface water of Ilam city
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Azadeh Ghadesi1   , Hamid Hasanpour1 , Razi Naserifar1 , Jahangir Abdi1 , Mohammad Reza Mahmoudi2 , Habibollah Turki3 , Omid Raiesi4 , Muhammad Ibrahim Getso5 , Asad Mirzaei * 6 |
1- Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran
2- Dept of Microbiology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran
3- Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
4- Student Research Committee, Ilam University of Medical Sciences, Ilam, Iran
5- Dept of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University Kano, PMB 3011, Kano, Nigeria
6- Zoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran & Dept of Parasitology and Mycology, School of Allied Medical Sciences, Ilam University of Medical Sciences, Ilam, Iran , mirzaeia421@gmail.com |
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Abstract: (158 Views) |
Acanthamoeba, a widely distributed free-living amoeba with 20 genotypes identified through rRNA gene sequencing, exhibits varying degrees of pathogenicity influenced by its genotype. This study focuses on assessing the prevalence of Acanthamoeba species in the surface waters of Ilam, located in western Iran, utilizing morphological analysis and sequencing of the 18S rRNA gene through the PCR method. A total of 50 water samples were collected from various regions within Ilam city, situated in the southwest of Iran. To isolate Acanthamoeba parasites from the samples, a culture method was used, and all utilized culture media were scrutinized through microscopic and molecular techniques. The parasite’s genotype was determined by sequencing a 500-bp fragment of the 18S rRNA gene. Using microscopic and molecular methods, 19 and 16 water samples tested positive, respectively. The 18S rRNA sequences revealed that the isolates belonged to the T4, T2, and T11 genotypes. This study emphasizes the presence and inclination for close contact with highly pathogenic genotypes of Acanthamoeba in the surface waters of Ilam City.
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| Keywords: Acanthamoeba, genotypes, Iran, water, 18srRNA |
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Full-Text [PDF 445 kb]
(99 Downloads)
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Type of Study: Research |
Subject:
medical parasitology
Received: 2024/10/5 | Accepted: 2024/10/5 | Published: 2024/09/22
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