Investigation of VEGF and FGF gene expression during angiogenesis of chick embryo chorioallantoic membrane treated with doxycycline antibiotic

Pourmohammad, Mohammadreza Pourmohammad; Khayatzadeh , Jina; Akbari , Seyyedeh Hananeh; Afsharanjad , Sima; Zafar Balanjad, Saeedeh

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Volume 32, Issue 5 (12-2024)

Journal of Ilam University of Medical Sciences 2024, 32(5): 80-91

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Investigation of VEGF and FGF gene expression during angiogenesis of chick embryo chorioallantoic membrane treated with doxycycline antibiotic

Mohammadreza Pourmohammad Pourmohammad¹

, Jina Khayatzadeh ^*

², Seyyedeh Hananeh Akbari³

, Sima Afsharanjad⁴

, Saeedeh Zafar Balanjad⁵

1- Dept of Medical Parasitology, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
2- Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran , J.khayatzadeh@mshdiau.ac.ir
3- Dept of Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
4- Dept of Biochemistry, Mashhad Medical Sciences Branch, Islamic Azad University, Mashhad, Iran
5- Dept of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract: (124 Views)

Introduction: Angiogenesis is the biological process of sprouting new vessels from existing vessels in the tissue. The main factors in the molecular guidance of this process are vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). Given the significance of antibiotics in reducing angiogenesis through the inhibition of collagenase, phospholipase A2, and several matrix metalloproteinases, the objective of this study was to examine the impact of the Doxycycline antibiotic on alterations in the gene expression of VEGF and FGF within the angiogenesis pathway of the chorioallantoic membrane (CAM) in chick embryos.
Materials & Methods: In this research, 50 Ross fertilized eggs were randomly divided into 5 groups, including the control group, laboratory control, and 3 experimental groups. On the eighth day of incubation, the laboratory control group was treated with normal saline, and the experimental groups were treated with doses of 50, 100, and 250 µmol/ml of the Doxycycline antibiotic. On the twelfth day, photographs were taken of the samples. The number and length of blood vessels and then the weight and length of embryos were measured. A real-time PCR technique was used to investigate the expression changes of VEGF and FGF genes on the CAM membrane. The statistical tests included one-way ANOVA, independent t-test, and post hoc tukey, which were analyzed by SPSS V.20 at a level of significance less than 0.05.
Results: The number of vessels and the length of vessels in the treatment groups showed a significant decrease compared to the control group (P<0.05). Also, the expression level of VEGF and FGF genes in the treatment groups was significantly lower than the control group (P<0.05).
Conclusion: The doxycycline antibiotic can significantly reduce the expression level of VEGF and FGF genes and the angiogenic process of the chorioallantoic membrane of chick embryos.

Keywords: Doxycycline antibiotic, Chorioallantoic Membrane, Angiogenesis, VEGF, FGF

Full-Text [PDF 1546 kb] (99 Downloads)

Type of Study: Research | Subject: biochemistry and nutrition
Received: 2024/04/18 | Accepted: 2024/08/7 | Published: 2024/12/5

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Pourmohammad M P, Khayatzadeh J, Akbari S H, Afsharanjad S, Zafar Balanjad S. Investigation of VEGF and FGF gene expression during angiogenesis of chick embryo chorioallantoic membrane treated with doxycycline antibiotic. J. Ilam Uni. Med. Sci. 2024; 32 (5) :80-91
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