The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility

Hamta, Ahmad; Bozorgi Moghadam, Zahra

doi:10.52547/sjimu.30.6.21

[Home ] [Archive]

[ فارسی ]

Journal of Ilam University of Medical Sciences

Main Menu

Home

Journal Information

Articles archive

Publication Ethics

Peer Review Process

Indexing Databases

For Authors

For Reviewers

Subscription

Contact us

Site Facilities

Google Scholar Metrics

	All	Since 2019
Citations	6308	3617
h-index	27	19
i10-index	185	78

Search in website

Receive site information

Registered in

Volume 30, Issue 6 (2-2023)

Journal of Ilam University of Medical Sciences 2023, 30(6): 21-31

Back to browse issues page

The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility

Ahmad Hamta ^*

¹, Zahra Bozorgi Moghadam²

1- Dept of biology, Faculty of science, Arak University, Arak, Iran , a-hamta@araku.ac.ir
2- Dept of biology, Faculty of science, Arak University, Arak, Iran

Abstract: (665 Views)

Introduction: Breast cancer is the most common malignancy in women worldwide. It is also the second leading cause of cancer death among women after lung cancer. Considering the relationship among plasma folate levels, the level of uracil, and DNA damage in cell division, methyl tetrahydrofolate reductase (MTHFR) is a suitable candidate for studies on the susceptibility to cancer, including breast cancer. This study aimed to investigate the relationship between two polymorphisms in the MTHFR gene and the risk of breast cancer among women in Markazi Province, Iran.
Material & Methods: This case-control study investigated the rs1801131 and rs1801133 polymorphisms of the MTHFR gene and breast cancer risk in a large population including 80 patients and 80 healthy women in Markazi Province, Iran, using the PCR-RFLP technique. The specimens were genotyped by agarose gel electrophoresis. Finally, the data were analyzed in SPSS software (version 26).
(Ethic code: Ir.arakmu.rec.1396.25)
Findings: There was a statistically significant difference between the two groups of patients and controls regarding three genotypes of the rs1801133 locus (P=0.006). Moreover, the CT genotype (P=0.001, OR=2.471, 95% CI=1.229-4.965) and total TT and CC genotypes (P=0.000, OR=3.375, 95% CI=1.716-6.637) were significantly associated with the risk of breast cancer. However, the TT genotype (P=0.538) showed a protective role against breast cancer. In contrast, there was no statistically significant difference between the two groups of patients and controls in terms of three genotypes of the rs1801131 locus (P=0.149), which is consistent with the results of some studies.
Discussion & Conclusion: The rs1801133 polymorphism of the MTHFR gene can be used as a marker in clinical prognostic studies related to the risk of breast cancer.

Keywords: Breast cancer, MTHFR, PCR-RFLP, Polymorphism, rs1801131, rs1801133

Full-Text [PDF 1088 kb] (308 Downloads)

Type of Study: Research | Subject: Molecular Genetics
Received: 2021/12/9 | Accepted: 2022/06/18 | Published: 2023/02/4

References

1. DeSantis C, Ma J, Bryan L, Jemal A. Breast cancer statistics, 2013. CA Cancer J Clin 2014; 64:52-62. doi: 10.3322/caac.21203.

2. Harbeck N, Penault-Llorca F, Cortes J, Gnant M, Houssami N, Poortmans P, et al. Breast cancer. Nat Rev Dis Primers 2019;5:66. doi: 10.1038/s41572-019-0111-2.

3. Waseem M, Hussain SR, Kumar S, Serajuddin M, Mahdi F, Sonkar SK, et al. Association of MTHFR (C677T) Gene Polymorphism with Breast Cancer in North India. Biomark Cancer 2016; 8:111-7. doi: 10.4137/BIC.S40446.

4. Ramos-Silva A, Figuera LE, Soto-Quintana OM, Puebla-Pérez AM, Ramírez-Patiño R, Gutiérrez-Hurtado I, et al. Association of the C677T polymorphism in the methylenetetra-hydrofolate reductase gene with breast cancer in a Mexican population. Genet Mol Res 2015; 14:4015-26. doi: 10.4238/2015.April.27.16.

5. Hosseini M, Houshmand M. MTHFR polymorphisms and breast cancer risk. Arch Med Sci 2010; 7, 1: 134-137. doi: 10.5114/

6. aoms.2011.20618.

7. Izmirli M. A literature review of MTHFR (C677T and A1298C polymorphisms) and cancer risk. Mol Biol Rep 2013;40:625-37. doi: 10.1007/s11033-012-2101-2.

8. Bravatà V. Controversial roles of methylenete-trahydrofolate reductase polymorphisms and folate in breast cancer disease. Int J Food Sci Nutr 2015;66:43-9. doi: 10.3109/09637486.2014.959896.

9. Akram M, Malik FA, Kayani MA. Mutational analysis of the MTHFR gene in breast cancer patients of Pakistani population. Asian Pac J Cancer Prev 2012;13:1599-603. doi: 10.7314/apjcp.2012.13.4.1599.

10. Li Z, Zhang J, Zou W, Xu Q, Li S, et al. The methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism is associated with breast cancer subtype susceptibility in south-western China. PLoS One 2021 9;16: e0254267. doi: 10.1371/journal.pone.0254267.

11. Hamzeie M, Abdolmaleki A, Zohre-Vand F, Khazayel S. Correlation of a well-known genetic variation in one-carbon metabolism pathway with breast cancer susceptibility in Iranian population. Cent Asian J Med Pharm Sci Innov 2021; 1;1:176-84. doi:10.22034/CAJMPSI.2021.04.02.

12. Rahimi Z, Bozorgi M, Rahimi Z, Shakiba E, Yari K, et al. MTHFR C677T Polymorphism Is Associated with the Risk of Breast Cancer Among Kurdish Population from Western Iran. Int J Cancer Manag 2019; 12:e67895. doi: 10.5812/ijcm.67895.

13. Yang M, Efehi N, Jin Y, Zhang Q, Ebadi AG, et al. Hot Water Extraction of Crude Polysaccharide from Codonopsis pilosula and Determination of the Rheological Properties. Rev Chim 2020; 71: 441-9. doi: 10.37358/RC.20.5.8155

14. Liang H, Yan Y, Li T, Li R, Li M, et al. Methylenetetrahydrofolate reductase poly-morphisms and breast cancer risk in Chinese population: a meta-analysis of 22 case–control studies. Tumor Biol 2014; 35:1695-701. doi: 10.1007/s13277-013-1234-9.

15. Bao S, Ebadi A, Toughani M, Dalle J, Maseleno A, et al. A new method for optimal parameters identification of a PEMFC using an improved version of Monarch Butterfly Optimization Algorithm. Int J Hydrogen Energy 2020; 45: 17882-92. doi: 10.1016/j.

16. ijhydene.2020.04.256.

17. Ibrahim NE, Ramadan SS. Strong Correlation of MTHFR Gene Polymorphisms with Breast Cancer and its Prognostic Clinical Factors among Egyptian Females. Asian Pac J Cancer Prev 2021;22:617-26. doi: 10.31557/APJCP.2021.22.2.617.

18. He L, Shen Y. MTHFR C677T polymorphism and breast, ovarian cancer risk: a meta-analysis of 19,260 patients and 26,364 controls. Onco Targets Ther 2017; 10:227-38. doi:10.2147/OTT.S121472.

19. Li Z, Zhang J, Zou W, Xu Q, Li S, et al. The methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism is associated with breast cancer subtype susceptibility in south-western China. Plos one 2021; 9;16:e0254267. doi: 10.1371/journal.pone.0254267.

20. Gimenez-Martins AP, Castanhole-Nunes MM, Nascimento-Filho CH, Santos SP, Galbiatti-Dias AL, et al. Association between folate metabolism polymorphisms and breast cancer: a case-control study. Genet Mol Biol 2021; 22; 44. doi:10.1590/1678-4685-GMB-2020-0485.

21. Lal H, Sharma B, Sambyal V, Guleria K, Singh NR, et al. Association of MTHFR 677C> T polymorphism with breast cancer risk: A case-control study and meta-analysis. J Cancer Res Ther 2022; 18:1451-60. doi: 10.4103/jcrt.JCRT_1063_20.

22. He JM, Pu YD, Wu YJ, Qin R, Zhang QJ, Sun YS, et al. Association between dietary intake of folate and MTHFR and MTR genotype with risk of breast cancer. Genet Mol Res 2014;13:8925-31. doi: 10.4238/2014.October.31.7.

23. Floris M, Sanna D, Castiglia P, Putzu C, Sanna V, et al. MTHFR, XRCC1 and OGG1 genetic polymorphisms in breast cancer: a case-control study in a population from North Sardinia. BMC cancer 2020; 20:1-5. doi: 10.1186/s12885-020-06749-w.

24. Ajaz S, Ali SM, Siddiqa A, Memon MA, Abid A, et al. Independent and Combined Associations of 677C/T and 1298A/C Polymorphisms in the MTHFR gene with Breast Cancers in a South-Asian Population. Medrxiv 2021; 03.13.21253512. doi:10.1101/2021.03.13.21253512.

25. Akram M, Malik FA, Kayani MA. Mutational analysis of the MTHFR gene in breast cancer patients of Pakistani population. Asian Pac J Cancer Prev 2012;13:1599-603. doi: 10.7314/apjcp.2012.13.4.1599.

Send email to the article author

Add your comments about this article

‎ 10.52547/sjimu.30.6.21

Ethics code: Ir.arakmu.rec.1396.25

Mendeley

Zotero

RefWorks

Hamta A, Bozorgi Moghadam Z. The Association of the MTHFR Gene Polymorphisms with Breast Cancer Susceptibility. J. Ilam Uni. Med. Sci. 2023; 30 (6) :21-31
URL: http://sjimu.medilam.ac.ir/article-1-7403-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 30, Issue 6 (2-2023)

Back to browse issues page

Persian site map - English site map - Created in 0.15 seconds with 41 queries by YEKTAWEB 4646