Phytochemical Investigation, Antioxidant Properties, and Identification
 of Organic and Inorganic Compounds from the Carob (Ceratonia siliqua L.) Fruit

Nasiri, Maryam; Mokhtari, Babak; Kolahi, Maryam; Pourreza, Nahid

doi:10.52547/sjimu.29.3.82

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Volume 29, Issue 3 (8-2021)

Journal of Ilam University of Medical Sciences 2021, 29(3): 82-91

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Phytochemical Investigation, Antioxidant Properties, and Identification of Organic and Inorganic Compounds from the Carob (Ceratonia siliqua L.) Fruit

Maryam Nasiri¹

, Babak Mokhtari¹

, Maryam Kolahi ^*

², Nahid Pourreza¹

1- Dept of Chemistry, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Dept of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran , m.kolahi@scu.ac.ir

Abstract: (1863 Views)

Introduction: Carob (Ceratonia siliqua L.) is a tree belonging to the Leguminosae family. It is used for forestry and ornamental purposes. The tree is of importance due to its medicinal properties and its ability to treat infertility. The present study aimed to investigate the chemical composition and fatty acid profile of the fruit.

Materials & Methods: Maximum extraction yields of the fruit were determined by utilizing different solvents for extraction. Methanolic extracts of the fruit were analyzed by GC-MS. EDS analysis was utilized for elemental analysis of the dried fruit powder (pods). The fatty acid profile of the fruit was determined by GC-FID. Antioxidant properties of the extracts were evaluated utilizing the ABTS assay.

Findings: Highest extraction yields were obtained with water. Phytochemical tests confirmed the presence of saponins, alkaloids, flavonoids, tannins, glycosides, steroids, and terpenoids in ethanolic extracts of the fruit. In total, 38 organic compounds were identified in methanolic extracts of the fruit, most of which belonged to fatty acids. Totally, 8 minerals were identified of which copper and zinc were the main minerals. Analysis of lipid extracts revealed the presence of eight fatty acids. Antioxidant activity of the extracts showed that aqueous extracts had the highest IC₅₀ value.

Discussions & Conclusions: The role of Carob fruit in the treatment of infertility can be due to the presence of many antioxidant compounds and their role in reducing oxidative stress in sperm and also the presence of nutrients as cofactors involved in the path of oxidative stress and also the presence of abundant fatty acids.

Keywords: Antioxidants, Carob, Chemical compounds, Fatty acids

Full-Text [PDF 509 kb] (638 Downloads)

Type of Study: Research | Subject: pharmacology
Received: 2020/05/31 | Accepted: 2021/03/13 | Published: 2021/09/1

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‎ 10.52547/sjimu.29.3.82

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Nasiri M, Mokhtari B, Kolahi M, Pourreza N. Phytochemical Investigation, Antioxidant Properties, and Identification of Organic and Inorganic Compounds from the Carob (Ceratonia siliqua L.) Fruit. J. Ilam Uni. Med. Sci. 2021; 29 (3) :82-91
URL: http://sjimu.medilam.ac.ir/article-1-6584-en.html

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