:: Volume 25, Issue 1 (5-2017) ::
Journal of Ilam University of Medical Sciences 2017, 25(1): 180-194 Back to browse issues page
Evaluation of Chemical Composition of Helichrysum artemisioides Extract Its Effect on Biofilm Formation and IcaD Gene Expression in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus
Amir Mirzaie * 1, Hassan Noorbazargan2 , Seyed Hamid Reza Khatami Khatami3 , Seyed Ataollah Sadat Shandiz4 , Arian Rahimi5 , Ali asghar Bagheri keshtali6
1- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran , Amir_mirzaie92@yahoo.com
2- Dept of Biotechnology, Faculty of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Dept of Bioscience and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran
4- Dept of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
5- Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
6- Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
Abstract:   (6622 Views)

Introduction: Staphylococcus aureus is a major causative agent of nosocomial infections which produces a wide range of diseases due to biofilm formation. This study aimed to investigate the phytochemical composition of Helichrysum artemisioides extract, its anti-biofilm effects on clinical isolates of ­methicillin-resistant Staphylococcus aureus, as well as, analysis of icaD gene expression.

Materials & methods: First, the phytochemical composition of H. artemisioides extract was studied by GC/MS method. Then, the biofilm formation capacity of 50 clinical isolates of S. aureus was evaluated by Congo red agar and polymerase chain reaction (PCR). Finally, after treatment of strains with SubMIC ­concentration of the extract within 24 hours, the expression level of icaD gene was evaluated by Real Time PCR.

Findings: The phytochemical analysis of extract showed 52 compounds, including α-Pinene (12.5%) and Carvacrol (8.6%) as the major components. The evaluation of biofilm formation test shows that out of 50 strains of S. aureus, 10 strains (20%) were positive for biofilms formation. Also, after treatment of selected strains by SubMIC concentration of the extract, the gene expression quantitation of icaD was down-regulated significantly (P<0.05).

Discusion & conclusions: Due to the significant effects of H. artemisioides extract on biofilm formation, it seems that the extract could be used as complementary therapy to inhibit S. aureus biofilm.

Keywords: Helichrysum artemisiodes, Staphylococcus areus, Biofilm, icaD
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Type of Study: Research |
Received: 2016/10/8 | Accepted: 2017/01/14 | Published: 2017/05/17

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