Introduction :  One the most prevalence  infection  is Urinary tracts  infection(UTI). Failure in diagnose  and treatment of UTI causes severe renal complications such as renal parenchyma damage,  hypertension and chronic renal failure .wasteful use of antibiotics were caused resistance to common drugs available.This study aims to investigate the pattern antibiotic resistance in bacterial strains isolated from urine of patients referred to clinical  laboratory of Ilam during 2012.

Materials & methods:  In a retrospective  study, data from portfolio patients referred to university hospitals and private laboratories  in  the city of  Ilam  were collected  by census sampling .  Spss software t-test and x² were used for analysing.

Findings: Escherichia coli (28.9%),  Staphylococcus aureus (28.8%), Staphylococcus saprophyticus (13%), Staphylococcus epidermidis (9%), Streptococcus. Spp(8%) and Enterococcus (7%)  were the most common pathogen. coterimoxazole and amikacin had the highest and lowest rates of antibiotic resistance.

Discussion & Conclusions: Result of this study indicated that E. coli  is the most common pathogen in patient  referred to laboratory and hospital of  Ilam city. The highest  resistant was observed for coterimoxazole . It is recommended that use of it should be limited.