Introduction: Echinococcosis disease is one of the most common diseases of human and animals in many parts of the world, including Iran, which is caused by larval stage of Echinococcus granulosus. This parasite has a high genetic diversity and including a complex of different genotypes (strains). Usually in areas where disease is endemic, there is a relatively high genetic diversity in parasite biologically. Studies in different parts of the world demonstrate that genotype variation and the nature of E.granulosus influenced on the life cycle of parasite, transmission routes, pathogenesis, and antigenicity, immunogenicity, response to medication, epidemiology and control of the disease. The aim of present study was to determination of genotype isolates of human and sheep hydatid in Ilam province for the first time. Materials & methods: 20 hydatid cyst samples were collected from sheep Ilam Industrial slaughterhouses and 10 human samples from Ilam hospitals. After DNA extraction of protoscoleces, mitDNA NADH dehydrogenase subunit 1 (nad-1) areas was amplified by PCR and the PCR products were analyzed by PCR-RFLP. Findings: Based on the results which obtained, the size of proliferative nad-1 products was 550 base pairs. Patterns of parts which obtained from PCR products after cutting by AluI and RsaI enzymes showed that all samples had a similar RFLP pattern but HpaII enzyme did not cut any region and had no change in fragment size. Discussion & Conclusion: The results of this study indicated that genotypic similarities between the size of DNA bands of E. granulosus from human and sheep isolates with PCR-RFLP method indicated the occurrence of similarities genotypes of E.granulosus in Ilam region. There is at least one genotype of parasite, which belongs to E. granulosous sensu stricto (G1-G3) complex.