1- Dept of Biology, Islamic Azad University, Kurdistan Science and Research Branch, Sanandaj, Iran 2- Dept of Biology, Islamic Azad University, Kurdistan Science and Research Branch, Sanandaj, Iran , gol.keshavarzi@gmail.com 3- Dept of Immunology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Abstract: (6243 Views)
Introduction: Type II diabetes is the most common form of diabetes and accounts for 90 percent of disease cases. CTLA4 represents a key regulatory element in the T cell/antigen-presenting cell interaction. Because CTLA4 involves in antigen-specific apoptosis and progressive failure of B-cell, a typical feature of type 2 diabetes, CTLA4 may be a candidate gene to indicate type 2 diabetes susceptibility. The aim of this study was to investigate the role of polymorphisms of CTLA-4 gene in type II diabetic patients compared with healthy subjects.
Materials & methods: In this case-control study, the blood samples were collected from 100 patients and 111 controls from Sanandaj, Hamedan and Kermanshah cities. After DNA extraction, the polymorphisms of CTLA-4 was investigated by ARMS-PCR method. Then, statistical analysis of results was performed using chi-square test.
Findings: The results show that the GG genotype at position +49 [OR (95% CI) = 1.92 P = 0.02] and in position - 318 TT and CT [OR (95% CI) = 9.80 P = 0.02] are a risk factor for Type II diabetes, while on position -1722 T/C [OR (95% CI) = 1.85 P =0.50] doesn’t have significant relationship.
Discussion & conclusions: These results suggest that the polymorphisms +49 A/G and -318 C/T of CTLA-4 gene have significant relationship with type II diabetes, without any significant relationship with the position of -1722 T/C.
KHadem pour S, keshavarzi F, Soulgi G. The Investigation of Relationship between Polymorphisms of CTLA-4geneand Type II Diabetes. J. Ilam Uni. Med. Sci. 2016; 24 (5) :22-30 URL: http://sjimu.medilam.ac.ir/article-1-2249-en.html