Introduction: Silicate nanoparticles are highly important in medical and treatment field due to their biocompatibility as well as ease of preparation and surface modif-ication. Through using various surface co-atings of nanoparticles, optimal and stable biomedical properties can be created for these nanoparticles. Therefore, this study aimed to evaluate the in vivo and in vitro login of these nanoparticles. Materials & Methods: In this study, Iodine-131 radiopharmaceutical stabilized by using silver on magnetite (Fe3O4) was used while synthesizing silicate magnetic nanoparticles including and it was stabilized in silicate nanoparticles. Then, entry of nanoparticles in MCF-7 breast cancer cell line and mouse was studied. Synthesis is done using sol-gel in the reverse microemulsion with Tetra-etoxy Silane reactive (TEOS) as the mon-omer, 3-amino propyl tre-autoxy silane (APTS) as monomer and the control agent of functional groups and zeta potential of nanoparticles.
 Findings: TEM results showed that the av-erage size of the nanoparticles was about 40 nm and about 80 percent of the primary iodine-131 was encapsulated in the nanop-articles. Also, the stability of nanoparticles was more than 99 percent in the carrier solution. Moreover, the highest entrance efficiency was 53-54% 2 to 4 hours after the beginning of cultivation.
Discussion & Conclusion: The prepared Silicate magnetic nanoparticles in this study can be used for simultaneous diagnosis and treatment of diseases.