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:: Volume 20, Issue 4 (2-2013) ::
sjimu 2013, 20(4): 176-181 Back to browse issues page
Isoelectric Focusing Optimization of Female Mouse Brain Protein Extract
A Meyfour 1, N Ahmadi, F Maleki
1- , meyfour_a@yahoo.com
Abstract:   (9953 Views)
Introduction: Brain (central nervous syst-em) is responsible to manage all biological process in living organisms, particularly mammalian. Since proteins play major roles in the biological cell activities, nowadays identification of brain proteins and deter-mination of their function is interesting subject for neurologists. Isoelectric Foc-using (IEF) technique is one of the prote-omic technologies to study brain proteins. This study was carried out to select the IPG strips with maximum resolution and to optimize IEF condition for the separation of mouse brain proteins. Materials & Methods: After extraction of mouse brain proteins and determination of protein concentration by Bradford assay, Isoelectric Focusing was performed using IPG strips with three different pH gradients. Findings: IPG strip with pH=3-10NL was able to separate more proteins with relati-vely high resolution in comparison with IPG strips with pH=3-10 and pH=4-7. Discussion & Conclusion: Results were shown that more proteins can be separated using IPG strip with pH=3-10 NL and this strip has a high resolution rather than the other strips. Due to the complexity of brain, its function, and the extent of brain proteins, it is appropriate to resolve proteins by the method.
Keywords: Mouse, Protein, Isoelectric fo-cusing, Brain
Full-Text [PDF 176 kb]   (2613 Downloads)    
Type of Study: Research | Subject: biology
Received: 2013/03/13 | Accepted: 2013/03/17 | Published: 2013/03/17
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meyfour A, ahmadi N, maleki F. Isoelectric Focusing Optimization of Female Mouse Brain Protein Extract. sjimu. 2013; 20 (4) :176-181
URL: http://sjimu.medilam.ac.ir/article-1-907-en.html


Volume 20, Issue 4 (2-2013) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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