[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 26, Issue 4 (11-2018) ::
sjimu 2018, 26(4): 43-52 Back to browse issues page
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
Rahele Naserian Moghadam Ms1, Fatemeh Roodbari2, Mahboubeh Naderi Nasab3, Davoud Mansouri3, Seyedeh Zohre Mirbagheri4, Aida Gholoobi5, Amin Hooshyar chichaklu3, Zahra Meshkat 6
1- Mazandaran University
2- Dept of Cell and Molecular Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran
3- Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4- Dept of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
5- Dept of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran , meshkatz@mums.ac.ir
Abstract:   (966 Views)

Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.
 
Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.
 
Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.
 
Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.

Keywords: Metallo-beta-lactamase, Pseudomonas aeruginosa, Antibiotic resistance, VIM gene
Full-Text [PDF 907 kb]   (250 Downloads)    
Type of Study: Research | Subject: exclusive bacteriology
Received: 2016/12/24 | Accepted: 2017/04/4
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naserian Moghadam R, Roodbari F, Naderi Nasab M, Mansouri D, Mirbagheri S Z, Gholoobi A, et al . The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa. sjimu. 2018; 26 (4) :43-52
URL: http://sjimu.medilam.ac.ir/article-1-4022-en.html


Volume 26, Issue 4 (11-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
Persian site map - English site map - Created in 0.21 seconds with 33 queries by YEKTAWEB 3960