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:: Volume 26, Issue 4 (11-2018) ::
sjimu 2018, 26(4): 43-52 Back to browse issues page
The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa
Rahele Naserian Moghadam Ms1, Fatemeh Roodbari 2, Mahboubeh Naderi Nasab 3, Davoud Mansouri 3, Seyedeh Zohre Mirbagheri 4, Aida Gholoobi 5, Amin Hooshyar chichaklu 3, Zahra Meshkat 6
1- Mazandaran University
2- Dept of Cell and Molecular Biology, Faculty of Basic Sciences, Mazandaran University, Mazandaran, Iran
3- Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4- Dept of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
5- Dept of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran , meshkatz@mums.ac.ir
Abstract:   (71 Views)

Introduction: Antibiotic resistance crisis has always been a serious problem for human health and many hospitalized patients are affected worldwide. Pseudomonas aeruginosa is a gram-negative pathogen and one of the most common causes of nosocomial infections. The main mechanism of resistance to beta-lactam antibiotics is the presence of metallo-beta-lactamase (MBL) enzymes. Most of the MBL genes are found in plasmids. The aim of this study was to evaluate the frequency of MBL-producing P. aeruginosa isolates caused by VIM-all and VIM 2, 3, 9, 11and16 genes.
 
Materials & Methods: Antimicrobial susceptibility of 127 clinical isolates of P. aeruginosa was determined using the standard Kirby-Bauer disk diffusion method according to Clinical and Laboratory Standard Institute (CLSI). Combined-disk test was used for phenotypic determination of MBLs-producing isolates. After DNA extraction, VIM-all and in specific, VIM 2, 3, 9, 11 and 16 genes were amplified using PCR method.
 
Findings: A total Of 127 clinical isolates of P. aeruginosa, 62 isolates (49%) were resistant to imipenem and 31 isolates (24.5%) showed phenotypic evidences of MBL production. Moreover, among imipenem resistant strains VIM-all genes were found in 12.5% of cases, but the VIM 2-3-9-11 and 16 genes were not detected in samples.
 
Discussion & Conclusions: The results obtained in this study suggest that in P. aeruginosa, the highest antibiotic resistance observed was to cefazolin (98%) followed by nalidixic acid (91%) and the least resistance were to ciprofloxacin (31%). One of the reasons for this trend is the growth of antibiotic-resistant bacteria and the known mechanisms of bacterial resistance.

Keywords: Metallo-beta-lactamase, Pseudomonas aeruginosa, Antibiotic resistance, VIM gene
Full-Text [PDF 907 kb]   (36 Downloads)    
Type of Study: Research | Subject: exclusive bacteriology
Received: 2016/12/24 | Accepted: 2017/04/4
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Naserian Moghadam R, Roodbari F, Naderi Nasab M, Mansouri D, Mirbagheri S Z, Gholoobi A, et al . The Frequency of VIM 2, 3, 9, 11 and VIM all among Metallo-beta-Lactamase Producing Pseudomonas aeruginosa. sjimu. 2018; 26 (4) :43-52
URL: http://sjimu.medilam.ac.ir/article-1-4022-en.html


Volume 26, Issue 4 (11-2018) Back to browse issues page
مجله علمی پزوهشی دانشگاه علوم پزشکی ایلام scientific journal of ilam university of medical sciences
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