[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
Publication Ethics::
Peer Review Process::
Indexing Databases::
For Authors::
For Reviewers::
Subscription::
Contact us::
Site Facilities::
::
Google Scholar Metrics

Citation Indices from GS

AllSince 2019
Citations62433553
h-index2719
i10-index18377

..
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Registered in

AWT IMAGE

AWT IMAGE

..
:: Volume 26, Issue 1 (5-2018) ::
Journal of Ilam University of Medical Sciences 2018, 26(1): 132-140 Back to browse issues page
Proteomics Comparison of Breast Cancer with Adjacent Normal Tissues in Women with Breast Cancer
Mayram Amiri Shoar1 , Masoumeh Hosseini2 , Ali Awsat Mellati * 3
1- Zanjan Metabolic Disease Research Center, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- مرکز تحقیقات بیماری ‌های متابولیک، دانشکده پزشکی، دانشگاه علوم پزشكي زنجان، زنجان، ایران
3- Zanjan Metabolic Disease Research Center, and Department of Biochemistry, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran , mellati3000@yahoo.com
Abstract:   (4487 Views)

Introduction: The molecular mechanisms involved in the development and progression of breast cancer have yet to be determined. In the present study, the proteome of cancerous beast and adjacent normal tissues were compared.
 
Materials & Methods: In a cohort study, the cancer tissues and adjacent normal tissues were obtained from 5 female patients with ductal carcinoma in stage 3. The total protein contents of cancer and adjacent normal tissues were extracted. The protein expression levels were examined by Image Master 2D Platinum software following two-dimensional gel electrophoresis. MALDI-TOF MS/MS mass spectrometry was used for proteins identification.
 
Findings: The constant region of Ig gamma-1 chain and beta subunit of hemoglobin were exclusively detected in the cancer and adjacent normal tissues, respectively. The expression of serum albumin and collagen VI alpha chain in the cancer tissue was significantly lower than the normal tissue (P <0.05). In contrast, the expression of a single peptide matching to cytoskeletal type I and II keratin significantly increased in the cancer tissue compared to the normal tissues (P <0.05).
 
Discussion & Conclusion: As the output of our investigation, it seems that proteome of cancerous tissue is extensively different from the adjacent one. Therefore, proteomic approach might be a promising tool for monitoring  breast tumorigenesis. However, this needs to be confirmed in future.

Keywords: biomarker, breast cancer, pathogenesis, proteom
Full-Text [PDF 743 kb]   (1965 Downloads)    
Type of Study: Research |
Received: 2016/03/13 | Accepted: 2017/02/4 | Published: 2018/05/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amiri Shoar M, Hosseini M, Awsat Mellati A. Proteomics Comparison of Breast Cancer with Adjacent Normal Tissues in Women with Breast Cancer . J. Ilam Uni. Med. Sci. 2018; 26 (1) :132-140
URL: http://sjimu.medilam.ac.ir/article-1-3443-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 26, Issue 1 (5-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی ایلام Journal of Ilam University of Medical Sciences
Persian site map - English site map - Created in 0.15 seconds with 40 queries by YEKTAWEB 4643